The inhibitory effect of glucose on phosphoenolpyruvate carboxykinase gene expression in cultured hepatocytes is transcriptional and requires glucose metabolism - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 1999

The inhibitory effect of glucose on phosphoenolpyruvate carboxykinase gene expression in cultured hepatocytes is transcriptional and requires glucose metabolism

Résumé

Phosphoenolpyruvate carboxykinase (PEPCK) is the rate-limiting enzyme of gluconeogenesis in the liver. PEPCK gene expression is controlled at the transcriptional level and is mainly regulated by hormones that are involved in glucose homeostasis. In this study, we have investigated the role of glucose on PEPCK gene expression in cultured hepatocytes. We demonstrate that glucose counteracts the stimulatory effect of glucocorticoids and cAMP on PEPCK expression. Glucose must be metabolized through glucokinase to have its inhibitory effect. The effect of glucose is mainly transcriptional and the region responsible for glucose inhibition is localized in the first 490 bp of the promoter.

Dates et versions

hal-02546617 , version 1 (18-04-2020)

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Fabienne Cournarie, Dalila D. Azzout-Marniche, Marc Foretz, Colette Guichard, Pascal Ferré, et al.. The inhibitory effect of glucose on phosphoenolpyruvate carboxykinase gene expression in cultured hepatocytes is transcriptional and requires glucose metabolism. FEBS Letters, 1999, 460 (3), pp.527-532. ⟨10.1016/s0014-5793(99)01407-6⟩. ⟨hal-02546617⟩
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