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Article Dans Une Revue Biochemical Engineering Journal Année : 2008

Effect of organic carbon sources and Fe2+ ions on growth and β-carotene accumulation by Dunaliella salina

Résumé

Effects of adding Fe2+ ions in photoheterotrophic cultivation of Dunaliella salina on growth and β-carotene synthesis were investigated. Different concentrations of Fe2+ ions and organic carbon source (acetate and malonate) were studied. A significant increase of cellular β-carotene content was observed, with a maximum value of 70 pg/cell in a culture enriched with 67.5 mM of acetate and 450 μM of FeSO4 and 33 pg/cell in the case of 67.5 mM of malonate and 450 μM of FeSO4. For comparison, the well-known nitrate starvation protocol was applied, and 35 pg/cell of β-carotene were obtained. By inducing an oxidative stress, Fe2+ ions revealed to stimulate β-carotene synthesis, especially when acting in the presence of the carbon source. Furthermore for some concentration of Fe2+ ions and organic carbon source, the loss in cells division was reduced compared to nitrate starvation.
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hal-02534067 , version 1 (17-04-2020)

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M. Mojaat, Jeremy Pruvost, A. Foucault, J. Legrand. Effect of organic carbon sources and Fe2+ ions on growth and β-carotene accumulation by Dunaliella salina. Biochemical Engineering Journal, 2008, 39 (1), pp.177-184. ⟨10.1016/j.bej.2007.09.009⟩. ⟨hal-02534067⟩
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