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Conference Papers Year : 2019

Development of Gold nanoparticles for the characterization of different bacteria by Surface Enhanced Raman Scattering

Abstract

Identification and quantification of microorganisms has become an essential issue in many areas like human health care and diagnostics, food and water safety and biodefense [1]. The conventional counting method, using plating and culturing, is the most straightforward and widely used method for this purpose but it appears tedious and time consuming. Since its discovery, Surface Enhanced Raman Spectroscopy (SERS) technique is increasingly used as faster and more convenient alternative. This technique allows to provide a high enhancement of Raman scattering from molecules adsorbed on a rough noble metal surface (silver, gold or copper). This phenomenon is explained by the combination of two well documented mechanisms: Electromagnetic effect (EE) and chemical effect (CE) [2]. The objective of our work was the development of gold nanoparticles (AuNps) as SERS substrate for the characterization of bacteria in order to avoid the use of the tiresome conventional counting method. The present study was divided in two parts. The first part consisted in developing the AuNPs synthesis method. Physico-chemical features of the synthetized AuNPs and their SERS efficiency using R6G model analyte have been investigated as a function of the synthesis experimental conditions. In the second part, the optimal operating conditions were used for bacteria characterization. The developed method was first tested for the quantification of Lactobacillus rhamnosus GG (LGG), a Gram-positive bacterium indicated for the treatment or the prevention of antibiotic-associated diarrhea. This strain being widely used in encapsulation systems, its characterization is required to determine encapsulation efficiency and bacteria release kinetics [3]. Then the potential of the developed method was investigated by characterizing other bacterial strains such as gram- negative bacterium Escherichia coli and gram-positive bacterium Bacillus subtilis.
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Dates and versions

hal-02519570 , version 1 (26-03-2020)

Identifiers

  • HAL Id : hal-02519570 , version 1

Cite

Elie Akanny, Anne Bonhommé, Laurence Bois, Carine Commun, Anne Doleans-Jordheims, et al.. Development of Gold nanoparticles for the characterization of different bacteria by Surface Enhanced Raman Scattering. Journées de Formulation 2019 : “Avancées dans la formulation de substances actives”, Jan 2019, Lyon, France. ⟨hal-02519570⟩
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