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Article Dans Une Revue Materials Today Communications Année : 2020

The effect of thermal treatment on the neuronal cell biocompatibility of SU-8

Résumé

Future in vitro life science studies that require in situ functionalities—going beyond the common glass petri dish—can potentially be realized by using microsystems technologies to fabricate bio-microelectromechanical systems (BioMEMS) and laboratory-on-a-chip (LOC). One such material used to construct these microsystems is the commercial photoresist SU-8. Here, the neuronal biocompatibility of SU-8 is examined as a function of its hard-baking thermal treatment. The SU-8 surfaces were non-patterned large uniform thin films—this allowed the biocompatibility and cell adhesion of the SU-8 to be fully tested. In total, 75 SU-8 samples were fabricated for the study using spin-coating and lithography techniques. We observe that a single hard-baking step of 180 °C for 2 h coupled with a coating (poly-d-lysine + laminin) is enough to detoxify SU-8 and promote primary cortical cell adhesion and survival up to 28 days in vitro (DIV). Therefore, the protocol described here makes SU-8 surfaces compatible with the development of neuronal networks from primary neural cells.
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Dates et versions

hal-02514939 , version 1 (06-01-2021)

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Tiffany Baëtens, Severine Begard, Emiliano Pallecchi, S. Arscott, V. Thomy, et al.. The effect of thermal treatment on the neuronal cell biocompatibility of SU-8. Materials Today Communications, 2020, pp.101073. ⟨10.1016/j.mtcomm.2020.101073⟩. ⟨hal-02514939⟩
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