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Chapitre D'ouvrage Année : 2019

Intact Protein Analysis by LC-MS for Characterizing Biomarkers in Cerebrospinal Fluid

Résumé

In the field of proteomics, the emerging "top-down" MS-based proteomics approach can be used to obtain a bird's eye view of all intact proteoforms present in a sample. This alternative to the "bottom-up" approach based on tryptic protein digestion processes has some unique advantages for assessing PTMs and sequence variations. However, it requires some dedicated tools for sample preparation and LC-MS analysis, which makes it more complex to handle than the bottom-up approach. In this study, a simple methodology is presented for characterizing intact proteins in biological fluid. This method yields quantitative information using an MS1 profiling approach and makes it possible to identify the proteins regulated under various clinical conditions.
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Dates et versions

hal-02505319 , version 1 (04-07-2020)

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Citer

Jérôme Vialaret, Sylvain Lehmann, Christophe Hirtz. Intact Protein Analysis by LC-MS for Characterizing Biomarkers in Cerebrospinal Fluid. Virginie Brun; Yohann Couté. Proteomics for Biomarker Discovery. Methods and Protocols, 1959, Springer, pp.163-172, 2019, Methods in Molecular Biology, 978-1-4939-9163-1. ⟨10.1007/978-1-4939-9164-8_11⟩. ⟨hal-02505319⟩
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