Highlights on the capacities of "Gel-based" proteomics - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proteome Science Année : 2010

Highlights on the capacities of "Gel-based" proteomics

François Chevalier

Résumé

Gel-based proteomic is the most popular and versatile method of global protein separation and quantification. This is a mature approach to screen the protein expression at the large scale, and a cheaper approach as compared with gelfree proteomics. Based on two independent biochemical characteristics of proteins, two-dimensional electrophoresis combines isoelectric focusing, which separates proteins according to their isoelectric point, and SDS-PAGE, which separates them further according to their molecular mass. The next typical steps of the flow of gel-based proteomics are spots visualization and evaluation, expression analysis and finally protein identification by mass spectrometry. For the study of differentially expressed proteins, two-dimensional electrophoresis allows simultaneously to detect, quantify and compare up to thousand protein spots isoforms, including post-translational modifications, in the same gel and in a wide range of biological systems. In this review article, the limits, benefits, and perspectives of gel-based proteomic approaches are discussed using concrete examples.
Fichier principal
Vignette du fichier
2010 Chevalier Article A1 PS.pdf (1.5 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02502775 , version 1 (26-02-2021)

Identifiants

Citer

François Chevalier. Highlights on the capacities of "Gel-based" proteomics. Proteome Science, 2010, 8 (1), pp.23. ⟨10.1186/1477-5956-8-23⟩. ⟨hal-02502775⟩
10 Consultations
24 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More