Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy. - Archive ouverte HAL Access content directly
Journal Articles Molecular Biology of the Cell Year : 2016

Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy.

François Aguet
  • Function : Author
Srigokul Upadhyayula
  • Function : Author
Yi-Ying Chou
  • Function : Author
Emanuele Cocucci
  • Function : Author
Kangmin He
  • Function : Author
Bi-Chang Chen
Kishore Mosaliganti
  • Function : Author
Mithun Pasham
  • Function : Author
Wesley Skillern
  • Function : Author
Wesley R Legant
  • Function : Author
Tsung-Li Liu
Greg Findlay
  • Function : Author
Eric Marino
  • Function : Author
Gaudenz Danuser
  • Function : Author
Sean Megason
  • Function : Author
Eric Betzig
Tom Kirchhausen
  • Function : Author

Abstract

Membrane remodeling is an essential part of transferring components to and from the cell surface and membrane-bound organelles and for changes in cell shape, which are particularly critical during cell division. Earlier analyses, based on classical optical live-cell imaging and mostly restricted by technical necessity to the attached bottom surface, showed persistent formation of endocytic clathrin pits and vesicles during mitosis. Taking advantage of the resolution, speed, and noninvasive illumination of the newly developed lattice light-sheet fluorescence microscope, we reexamined their assembly dynamics over the entire cell surface and found that clathrin pits form at a lower rate during late mitosis. Full-cell imaging measurements of cell surface area and volume throughout the cell cycle of single cells in culture and in zebrafish embryos showed that the total surface increased rapidly during the transition from telophase to cytokinesis, whereas cell volume increased slightly in metaphase and was relatively constant during cytokinesis. These applications demonstrate the advantage of lattice light-sheet microscopy and enable a new standard for imaging membrane dynamics in single cells and multicellular assemblies.

Dates and versions

hal-02482289 , version 1 (17-02-2020)

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Cite

François Aguet, Srigokul Upadhyayula, Raphaël Gaudin, Yi-Ying Chou, Emanuele Cocucci, et al.. Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy.. Molecular Biology of the Cell, 2016, 27 (22), ⟨10.1091/mbc.E16-03-0164⟩. ⟨hal-02482289⟩

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