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Communication Dans Un Congrès Année : 2015

Neurostimulation with nanosecond pulsed electric fields: A combined electrophysiology and multiphoton imaging approach in intact ganglia

Sergii Romanenko
  • Fonction : Auteur
Delia Arnaud-Cormos
OSA
Sylvia M. Bardet
Philippe Lévêque
OSA
Rodney P O'Connor
  • Fonction : Auteur

Résumé

The effect of high power nanosecond pulsed electric fields (nsPEF) was examined on the complex neuronal assemblies of intact leech ganglia. The medicinal leech midbody ganglion is a convenient experimental preparation that represents a real neuronal network with neuronal diversity and glial cells. 10 ns pulses of different intensities ranging from 22.5 to 47 kV/cm were applied to the ganglia body and alterations in natural electrical activity of Retzius cells were recorded with sharp glass electrode in a current-clamp mode or multiphoton whole-ganglia imaging with calcium indicators. The lowest nsPEF intensity tested resulted in stimulation of a single action potential (AP) whereas all higher intensities used led to bursts of APs.
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Dates et versions

hal-02469315 , version 1 (17-11-2020)

Identifiants

  • HAL Id : hal-02469315 , version 1

Citer

Sergii Romanenko, Delia Arnaud-Cormos, Sylvia M. Bardet, Philippe Lévêque, Rodney P O'Connor. Neurostimulation with nanosecond pulsed electric fields: A combined electrophysiology and multiphoton imaging approach in intact ganglia. BIOEM, BEMS AND EBEAM, 2015, ASILOMAR, United States. ⟨hal-02469315⟩
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