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Article Dans Une Revue FEBS Letters Année : 1992

Human centrosomal epitope is shared specifically with human lactate dehydrogenase-B isozyme

Françoise Gosti
S.S.L. Li
  • Fonction : Auteur
R. Maunoury
  • Fonction : Auteur
M. Bornens
  • Fonction : Auteur

Résumé

A rabbit serum (0013) used to identify pericentriolar proteins from isolated centrosomes (Gosti-Testu, F., Marty, M.C., Berges, J., Maunoury, R. and Bornens, M. (1986) EMBO J. 5, 2545 2550) was shown also to react through the same epitope with several non-centrosomal proteins including a major 36 kDa cytosolic antigen. This protein was identified to be human lactate dehydrogenase and the co-distribution of 0013 epitope on the centrosomal protein and on lactate dehydrogenase (LDH) was shown to be specific for human cells (Gosti, F., Marty, M.C., Courvalin, J.C., Maunoury, R. and Bornens, M. (1987) Proc. Natl. Acad. Sci. USA 84, 1000-1004). Human hepatic cells constitute, so far, the only exception to this co-distribution rule. By using this cell type which expresses only the LDH-A4 isozyme, we demonstrate that 0013 epitope is specific for the human LDH-B subunit, making serum 0013 the strongest anti-LDH-B available so far. The evolutionary and physiological significance of this situation is discussed.

Dates et versions

hal-02447057 , version 1 (21-01-2020)

Identifiants

Citer

Françoise Gosti, S.S.L. Li, R. Maunoury, M. Bornens. Human centrosomal epitope is shared specifically with human lactate dehydrogenase-B isozyme. FEBS Letters, 1992, 299 (3), pp.231-234. ⟨10.1016/0014-5793(92)80121-V⟩. ⟨hal-02447057⟩
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