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Article Dans Une Revue Plasmid Année : 1983

Restriction map of Tn7

Résumé

Tn7, a transposon of 14 kb, encodes resistance to trimethoprim (Tp) and streptomycin (Sm). A cleavage site map of this transposon for twenty-two different restriction enzymes as determined by comparison of restriction enzyme cleavage patterns of the plasmids ColE1 and ColE1::Tn7 is presented. The precise localization of these sites was facilitated by the use of two deletion derivatives of ColE1::Tn7: pGB2 and ColE1::Tn7 delta 6, and by the use of pOB14 and pOB15 which contain a part of Tn7 cloned into the plasmid pBR322. This map should aid in the study of the structural and genetic organization of this transposon.

Dates et versions

hal-02446818 , version 1 (21-01-2020)

Identifiants

Citer

Françoise Gosti-Testu, Victor Norris, Jean Brevet. Restriction map of Tn7. Plasmid, 1983, 10 (1), pp.96-99. ⟨10.1016/0147-619x(83)90061-6⟩. ⟨hal-02446818⟩
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