Coniacian to Maastrichtian changes in calcareous nannofossil assemblages have been investigated in the eastern Tethyan Shahneshin section (central Zagros Basin, Iran). The nannofossil assemblages are mainly composed of Watznaueria spp. (avg. 54%), Retecapsa spp (avg. 7.9%), Cribrosphaerella ehrenbergii (avg. 7.7%) and Micula spp. (avg. 5.7%). Throughout the late Campanian, there is a trend to lower abundances in Watznaueria spp. together with increasing abundances of C. ehrenbergii and Arkhangelskiella cymbiformis, which are considered in this basin as the main cool-water taxa. Our results reveal that, despite a diagenetic impact on calcareous nannoflora, a number of primary paleoecological trends are preserved which depict well features of the progressive Late Cretaceous cooling. The first pronounced cooling episode occurs across the late Campanian to early Maastrichtian. The onset of pronounced cooling in the eastern Tethys appears to occur prior to the Campanian/Maastrichtian Boundary event (CMBE) δ13C negative excursion, in contrast with the Boreal realm where pronounced cooling only occurs in the early Maastrichtian, postdating the onset of the CMBE. The coincidence of this earlier cooling in the Zagros Basin with an interval characterized by a significant increase in benthic foraminifera suggests an amplified response of the assemblage due to a change to shallower environments. Hence, the late Campanian calcareous nannofossil assemblage turnover in central Zagros is either a response to an early cooling trend in the eastern Tethys or to sea-level fall or both. The mid-Maastrichtian warming and late Maastrichtian cooling episodes are also delineated in the nannofossil assemblage of Shahneshin and likely correlate with similar episodes in the Boreal Realm.