p21WAF-1 reorganizes the nucleus in tumor suppression - Archive ouverte HAL Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 1998

p21WAF-1 reorganizes the nucleus in tumor suppression

G. Linares-Cruz
  • Function : Author
H. Bruzzoni-Giovanelli
  • Function : Author
V. Alvaro
  • Function : Author
J.-P. Roperch
  • Function : Author
M. Tuynder
  • Function : Author
M. Nemani
  • Function : Author
F. Lethrosne
  • Function : Author
L. Piouffre
  • Function : Author
V. Reclar
  • Function : Author
A. Faille
  • Function : Author
D. Chassoux
  • Function : Author
R. Amson
  • Function : Author
Adam Telerman
  • Function : Author
  • PersonId : 1060979

Abstract

Interphasic nuclear organization has a key function in genome biology. We demonstrate that p21WAF-1, by influencing gene expression and inducing chromosomal repositioning in tumor suppression, plays a major role as a nuclear organizer. Transfection of U937 tumor cells with p21WAF-1 resulted in expression of the HUMSIAH (human seven in absentia homologue), Rb, and Rbr-2 genes and strong suppression of the malignant phenotype. p21(WAF-1) drastically modified the compartmentalization of the nuclear genome. DNase I genome exposure and fluorescence in situ hybridization show, respectively, a displacement of the sensitive sites to the periphery of the nucleus and repositioning of chromosomes 13, 16, 17, and 21. These findings, addressing nuclear architecture modulations, provide potentially significant perspectives for the understanding of tumor suppression.

Dates and versions

hal-02412855 , version 1 (15-12-2019)

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G. Linares-Cruz, H. Bruzzoni-Giovanelli, V. Alvaro, J.-P. Roperch, M. Tuynder, et al.. p21WAF-1 reorganizes the nucleus in tumor suppression. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95 (3), pp.1131-1135. ⟨10.1073/pnas.95.3.1131⟩. ⟨hal-02412855⟩
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