Molecular architecture of bacterial amyloids in Bacillus biofilms - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FASEB Journal Année : 2019

Molecular architecture of bacterial amyloids in Bacillus biofilms

Jesus Hierrezuelo
  • Fonction : Auteur
Jesús Cámara-Almirón
  • Fonction : Auteur
Joaquín Caro-Astorga
  • Fonction : Auteur
Ana Álvarez-Mena
  • Fonction : Auteur
Antoine Dutour
  • Fonction : Auteur
Jayakrishna Shenoy
  • Fonction : Auteur
Estelle Morvan
Brice Kauffmann

Résumé

The formation of biofilms provides structural and adaptive bacterial response to the environment. In Bacillus species, the biofilm extracellular matrix is composed of exopolysaccharides, hydrophobins, and several functional amyloid proteins. We report, using multiscale approaches such as solid-state NMR (SSNMR), electron microscopy, X-ray diffraction, dynamic light scattering, attenuated total reflection Fourier transform infrared (FTIR), and immune-gold labeling, the molecular architecture of B. subtilis and pathogenic B. cereus functional amyloids. SSNMR data reveal that the major amyloid component TasA in its fibrillar amyloid form contain beta-sheet and alpha-helical secondary structure, suggesting a nontypical amyloid architecture in B. subtilis. Proteinase K digestion experiments indicate the amyloid moiety is 100 aa long, and subsequent SSNMR and FTIR signatures for B. subtilis and B. cereus TasA filaments highlight a conserved amyloid fold, albeit with substantial differences in structural polymorphism and secondary structure composition. Structural analysis and coassembly data on the accessory protein TapA in B. subtilis and its counterpart camelysin in B. cereus reveal a catalyzing effect between the functional amyloid proteins and a common structural architecture, suggesting a coassembly in the context of biofilm formation. Our findings highlight nontypical amyloid behavior of these bacterial functional amyloids, underlining structural variations between biofilms even in closely related bacterial species.-El Mammeri, N., Hierrezuelo, J., Tolchard, J., Camara-Almiron, J., Caro-Astorga, J., Alvarez-Mena, A., Dutour, A., Berbon, M., Shenoy, J., Morvan, E., Grelard, A., Kauffmann, B., Lecomte, S., de Vicente, A., Habenstein, B., Romero, D., Loquet, A. Molecular architecture of bacterial amyloids in Bacillus biofilms.

Domaines

Matériaux
Fichier non déposé

Dates et versions

hal-02412754 , version 1 (23-09-2020)

Identifiants

Citer

Nadia El Mammeri, Jesus Hierrezuelo, James Tolchard, Jesús Cámara-Almirón, Joaquín Caro-Astorga, et al.. Molecular architecture of bacterial amyloids in Bacillus biofilms. FASEB Journal, In press, 33 (11), pp.fj201900831R-fj201900831R. ⟨10.1096/fj.201900831R⟩. ⟨hal-02412754⟩

Collections

INC-CNRS
52 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More