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                <term xml:lang="en">Spectroscopy</term>
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                <term xml:lang="en">cytochrome b5</term>
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              <p>This article details the surface-enhanced Raman scattering (SERS) performance of plasmonicsubstrates fabricated by a physical metal evaporation technique that uses no precursor orintermediate coating. We outline a cost-effective nanofabrication protocol that uses commonlaboratory equipment to produce homogeneously covered crystalline nanoparticle substrates.Our fabrication yields a homogeneous SERS response over the whole surface. The platform istested with methylene blue diluted at various concentrations to estimate the sensitivity,homogeneity, and reproducibility of the process. The capacity of the substrates is also confirmedwith spectroscopic investigations of human microsomal cytochrome b5.</p>
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