A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons - Archive ouverte HAL Access content directly
Journal Articles Nucleic Acids Research Year : 2020

A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons

Marc Haenlin

Abstract

The histone demethylase LSD1 is a key chromatin regulator that is often deregulated in cancer. Its or-tholog, dLsd1 plays a crucial role in Drosophila oo-genesis; however, our knowledge of dLsd1 function is insufficient to explain its role in the ovary. Here, we have performed genome-wide analysis of dLsd1 binding in the ovary, and we document that dLsd1 is preferentially associated to the transcription start site of developmental genes. We uncovered an unan-ticipated interplay between dLsd1 and the GATA transcription factor Serpent and we report an unexpected role for Serpent in oogenesis. Besides, our transcrip-tomic data show that reducing dLsd1 levels results in ectopic transposable elements (TE) expression correlated with changes in H3K4me2 and H3K9me2 at TE loci. In addition, our results suggest that dLsd1 is required for Piwi dependent TE silencing. Hence, we propose that dLsd1 plays crucial roles in establishing specific gene expression programs and in repressing transposons during oogenesis.
Fichier principal
Vignette du fichier
gkz1142.pdf (6.64 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02395290 , version 1 (05-12-2019)

Identifiers

Cite

Julie M J Lepesant, Carole Iampietro, Eugenia Galeota, Benoit Augé, Marion Aguirrebengoa, et al.. A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons. Nucleic Acids Research, 2020, 38 (3), pp.1206-1224. ⟨10.1093/nar/gkz1142⟩. ⟨hal-02395290⟩
134 View
24 Download

Altmetric

Share

Gmail Facebook X LinkedIn More