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Article Dans Une Revue Chemical Communications Année : 2016

Direct conjugation of antibodies to the ZnS shell of quantum dots for FRET immunoassays with low picomolar detection limits

Résumé

Compact and functional nanoparticle-antibody conjugates are of paramount importance for the development of quantum dot (QD)-based immunoassays. Here, we present a simple strategy to directly conjugate IgG, F(ab')2, and Fab antibodies via their endogenous disulfide groups directly to the inorganic ZnS shell of compact penicillamine-coated QDs. The functionality of the conjugates was demonstrated by terbium (Tb)-to-QD FRET immunoassays against prostate specific antigen in serum samples. Detection limits of 2.5 pM (0.080 ng mL(-1)) were 10 and 25 times lower compared to conjugation via maleimide-terminated ligands and polymer chains, respectively. These more compact, simple, and sensitive QD-antibody conjugates will be highly advantageous for nanocrystal-based biosensing applications.
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Dates et versions

hal-02393613 , version 1 (04-12-2019)

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Citer

S. Bhuckory, L. Mattera, K. D. Wegner, X. Qiu, Y.-T. Wu, et al.. Direct conjugation of antibodies to the ZnS shell of quantum dots for FRET immunoassays with low picomolar detection limits. Chemical Communications, 2016, 52 (100), pp.14423--14425. ⟨10.1039/c6cc08835j⟩. ⟨hal-02393613⟩
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