TM8 represses developmental timing in Nicotiana benthamiana and has functionally diversified in angiosperms - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue BMC Plant Biology Année : 2018

TM8 represses developmental timing in Nicotiana benthamiana and has functionally diversified in angiosperms

Heleen Coenen
  • Fonction : Auteur
Tom Viaene
  • Fonction : Auteur
Koen Geuten
  • Fonction : Auteur

Résumé

Background: MADS-box genes are key regulators of plant reproductive development and members of most lineages of this gene family have been extensively studied. However, the function and diversification of the ancient TM8 lineage remains elusive to date. The available data suggest a possible function in flower development in tomato and fast evolution through numerous gene loss events in flowering plants. Results: We show the broad conservation of TM8 within angiosperms and find that in contrast to other MADS-box gene lineages, no gene duplicates have been retained after major whole genome duplication events. Through knock-down of NbTM8 by virus induced gene silencing in Nicotiana benthamiana, we show that NbTM8 represses miR172 together with another MADS-box gene, SHORT VEGETATIVE PHASE (NbSVP). In the closely related species Petunia hybrida, PhTM8 is not expressed under the conditions we investigated and consistent with this, a knock-out mutant did not show a phenotype. Finally, we generated transgenic tomato plants in which TM8 was silenced or ectopically expressed, but these plants did not display a clear phenotype. Therefore, no clear function could be confirmed for Solanum lycopersium. Conclusions: While the presence of TM8 is generally conserved, it remains difficult to propose a general function in angiosperms. Based on all the available data to date, supplemented with our own results, TM8 function seems to have diversified quickly throughout angiosperms and acts as repressor of miR172 in Nicotiana benthamiana, together with NbSVP.
Fichier principal
Vignette du fichier
2018_Coenen_BMC Plant_Biol_1.pdf (7.06 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02389867 , version 1 (26-05-2020)

Licence

Paternité

Identifiants

Citer

Heleen Coenen, Tom Viaene, Michiel Vandenbussche, Koen Geuten. TM8 represses developmental timing in Nicotiana benthamiana and has functionally diversified in angiosperms. BMC Plant Biology, 2018, 18 (1), ⟨10.1186/s12870-018-1349-7⟩. ⟨hal-02389867⟩
49 Consultations
34 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More