Thermal imaging and the recent availability of widely tunable infrared QCL lasers (Quantum Cascade Laser) allow us to propose an active hyperspectral imaging system operating in mid-infrared (MIR) band to obtain simultaneously large amounts of spatial and spectral information on the samples. In order to evaluate more precisely the capacities of the active hyperspectral imaging, we propose a system composed of four powerful QCL tunable lasers (in order to cover 3-5 μm and 7-11 μm wavelengths) and three cameras: a visible and near-infrared (NIR) range, a bolometer for 7-13 μm range and an InSb cooled camera for 3-5 μm range. We present the algorithm for image acquisition, image and data processing. Finally, we present and discuss some preliminary results using this system to characterize plant leaves under controlled growing conditions.