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Article Dans Une Revue International Journal of Molecular Sciences Année : 2019

Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide

Résumé

Cell-penetrating peptides (CPPs) are short peptides that can translocate and transport cargoes into the intracellular milieu by crossing biological membranes. The mode of interaction and internalization of cell-penetrating peptides has long been controversial. While their interaction with anionic membranes is quite well understood, the insertion and behavior of CPPs in zwitterionic membranes, a major lipid component of eukaryotic cell membranes, is poorly studied. Herein, we investigated the membrane insertion of RW16 into zwitterionic membranes, a versatile CPP that also presents antibacterial and antitumor activities. Using complementary approaches, including NMR spectroscopy, fluorescence spectroscopy, circular dichroism, and molecular dynamic simulations, we determined the high-resolution structure of RW16 and measured its membrane insertion and orientation properties into zwitterionic membranes. Altogether, these results contribute to explaining the versatile properties of this peptide toward zwitterionic lipids.
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Dates et versions

hal-02380921 , version 1 (21-12-2020)

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Marie-Lise Jobin, Lydie Vamparys, Romain Deniau, Axelle Grélard, Cameron Mackereth, et al.. Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide. International Journal of Molecular Sciences, 2019, 20 (18), pp.4441. ⟨10.3390/ijms20184441⟩. ⟨hal-02380921⟩
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