A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis - Archive ouverte HAL Access content directly
Journal Articles New Phytologist Year : 2014

A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis

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Thomas Blein
Linnan Ma
  • Function : Author
Maria Fransiska Njo
  • Function : Author
Tom Beeckman
Martin Crespi
Caroline Hartmann
  • Function : Author
  • PersonId : 1202639

Abstract

In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants. By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching. We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels. We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis.
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Dates and versions

hal-02379657 , version 1 (25-11-2019)

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Céline Sorin, Marie Declerck, Aurélie Christ, Thomas Blein, Linnan Ma, et al.. A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis. New Phytologist, 2014, 202 (4), pp.1197-1211. ⟨10.1111/nph.12735⟩. ⟨hal-02379657⟩
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