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Article Dans Une Revue BBA - Gene Structure and Expression Année : 1991

Functional expression of urea channels in amphibian oocytes injected with frog urinary bladder mRNA

Résumé

In amphibian urinary bladder epithelium, vasopressin increases passive urea permeability, concomitant with the appearance of a facilitated urea transport. Amphibian oocytes from Xenopus laevis and Rana esculenta microinjected with total or fractionated poly(A +) RNA isolated from frog urinary bladder epithellal cells. After several (3-5) days at 18°C, the urea flux was assayed by measuring the uptake and efflux of [14C] urea in water-injected and mRNA-injected oocytes. A 2 to 3-fold increase of urea transport was detected in oocytes injected either with total mRNA or with a 6-10 kilobase mRNA fraction, when compared with water-injected oocytes. This expression of urea channels was inhibited by 0.1 mM phloretin (50% inhibition) and 0.1 mM nitrophenylthiourea (up to 70% inhibition). On the contrary, no expression was detected in brain mRNA-injected oocytes. These results show the specific functional expression of the phloretin and NPTU-sensitive urea channel (or carrier), from frog urinary bladder epithelial cells, providing an approach for the expression cloning of these urea channels.

Dates et versions

hal-02376500 , version 1 (27-11-2019)

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Citer

Sonia Martial, Pierre Ripoche, Cristina Ibarra. Functional expression of urea channels in amphibian oocytes injected with frog urinary bladder mRNA. BBA - Gene Structure and Expression, 1991, 1090 (1), pp.86-90. ⟨10.1016/0167-4781(91)90040-S⟩. ⟨hal-02376500⟩
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