Regulation of Aldose Reductase, Sorbitol Dehydrogenase, and Taurine Cotransporter mRNA in Rat Medulla - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of the American Society of Nephrology Année : 1995

Regulation of Aldose Reductase, Sorbitol Dehydrogenase, and Taurine Cotransporter mRNA in Rat Medulla

Sonia Martial
S Russ Price
  • Fonction : Auteur
Jeff Sands
  • Fonction : Auteur

Résumé

The regulation of mRNA for aldose reductase, sorbitol dehydrogenase, and the Na+/Cl-/taurine cotransporter was studied with three in vivo models in which urinary concentration is reduced: Sprague-Dawley rats undergoing a water diuresis or fed a low-protein diet or Brattleboro rats. In Sprague-Dawley rats, 3 days of water diuresis reduced inner medullary aldose reductase mRNA abundance 6.5-fold compared with untreated rats, whereas sorbitol dehydrogenase and taurine cotransporter mRNA were unchanged. When water diuretic rats were acutely deprived of water, urine osmolality increased significantly after 4 h but aldose reductase mRNA did not increase until 12 h. Heat shock protein-70 mRNA was not increased by water deprivation. Second, in rats fed a low-protein diet for 3 wk, aldose reductase mRNA increased two-fold, whereas sorbitol dehydrogenase and taurine cotransporter mRNA were unchanged. Finally, in Brattleboro rats, urine osmolality and levels of aldose reductase and taurine cotransporter mRNA increased in response to 1 day of water deprivation, whereas sorbitol dehydrogenase mRNA was unchanged. Administering vasopressin (1 U/day) to Brattleboro rats for 8 days also increased urine osmolality and aldose reductase mRNA but did not alter sorbitol dehydrogenase or taurine cotransporter mRNA. This result is consistent with the hypothesis that changes in urine osmolality induce changes in aldose reductase mRNA abundance that are independent of vasopressin. It was concluded that, in rat inner medulla: (1) aldose reductase mRNA abundance varies with changes in water balance or dietary protein, whereas sorbitol dehydrogenase and taurine cotransporter mRNA do not; and (2) heat shock protein-70 mRNA abundance is not increased during acute osmotic stress.
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Dates et versions

hal-02376367 , version 1 (27-11-2019)

Identifiants

  • HAL Id : hal-02376367 , version 1
  • PUBMED : 7620095

Citer

Sonia Martial, S Russ Price, Jeff Sands. Regulation of Aldose Reductase, Sorbitol Dehydrogenase, and Taurine Cotransporter mRNA in Rat Medulla. Journal of the American Society of Nephrology, 1995, 5 (11), pp.1971-8. ⟨hal-02376367⟩
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