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Article Dans Une Revue Methods in Molecular Biology Année : 2019

Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy

Résumé

Supported lipid bilayers represent a very attractive way to mimic biological membranes, especially to investigate molecular mechanisms associated with the lateral segregation of membrane components. Observation of these model membranes with high-speed atomic force microscopy (HS-AFM) allows the capture of both topography and dynamics of membrane components, with a spatial resolution in the nanometer range and image capture time of less than 1 s. In this context, we have developed new protocols adapted for HS-AFM to form supported lipid bilayers on small mica disks using the vesicle fusion or Langmuir-Blodgett methods. In this chapter we describe in detail the protocols to fabricate supported artificial bilayers as well as the main guidelines for HS-AFM imaging of such samples.
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Dates et versions

hal-02373440 , version 1 (10-11-2021)

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Hussein Nasrallah, Anthony Vial, Nicolas Pocholle, Jérémy Soulier, Luca Costa, et al.. Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy. Methods in Molecular Biology, 2019, pp.45-59. ⟨10.1007/978-1-4939-8894-5_3⟩. ⟨hal-02373440⟩
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