Chapitre D'ouvrage Année : 2019

Simple Protocol to Purify Cell Wall Polysaccharide from Gram-Positive Bacteria and Assess Its Structural Integrity

Résumé

Cell wall polysaccharides (CWPS), which are usually covalently bound to the peptidoglycan and are closely associated with the cell wall, are considered as ubiquitous components of the cell envelope of gram-positive bacteria and play an important role as mediators of bacterial interactions with the environment. Here, we describe a simple method for purifying CWPS by extraction of bacterial cells with consecutive acid treatments. Purified CWPS are obtained by gel-filtration chromatography following treatment with HF. We also provide the methodology to easily assess the integrity of CWPS using high-resolution magic-angle spinning (HR-MAS) NMR.

Dates et versions

hal-02371889 , version 1 (20-11-2019)

Identifiants

Citer

Irina Sadovskaya, Yann Guerardel. Simple Protocol to Purify Cell Wall Polysaccharide from Gram-Positive Bacteria and Assess Its Structural Integrity. Inka Brockhausen. Bacterial Polysaccharides Methods and Protocols, 1954, Humana New York, pp.37-45, 2019, Methods in Molecular Biology (MIMB,volume 1954), 978-1-4939-9153-2. ⟨10.1007/978-1-4939-9154-9_4⟩. ⟨hal-02371889⟩
107 Consultations
0 Téléchargements

Altmetric

Partager

  • More