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Article Dans Une Revue Anal Biochem Année : 1988

Zinc-binding proteins detected by protein blotting

Résumé

The Western blotting technique was used for the detection of zinc-binding proteins. Proteins were separated electrophoretically on 15% polyacrylamide-sodium dodecyl sulfate minigels, the gels were soaked in a reduction buffer, and the proteins were transferred to nitrocellulose filters. Zinc-binding proteins were probed with radioactive zinc (65Zn) and were detected by autoradiography. This technique allows the detection of as little as 20 to 100 pmol of zinc metalloproteins.

Dates et versions

hal-02371456 , version 1 (19-11-2019)

Identifiants

Citer

A Mazen, G Gradwohl, G De Murcia. Zinc-binding proteins detected by protein blotting. Anal Biochem, 1988, 172 (1), pp.39-42. ⟨10.1016/0003-2697(88)90408-3⟩. ⟨hal-02371456⟩

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