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Article Dans Une Revue Biochem Biophys Res Commun Année : 1987

Poly(ADP-ribose) polymerase forms loops with DNA

Résumé

The interaction between highly purified poly(ADP-ribose) polymerase from calf thymus and different topological forms of pBR322 DNA has been studied by gel retardation electrophoresis and electron microscopy. We show that: (i) in the absence of nicks on DNA the enzyme has a marked affinity for supercoiled (form I) DNA, (ii) in the presence of single stranded breaks poly(ADP-ribose) polymerase preferentially binds to form II, (iii) in all cases enzyme molecules are frequently located at DNA intersections, (iv) a cooperative binding of the enzyme on DNA occurs.

Dates et versions

hal-02371453 , version 1 (19-11-2019)

Identifiants

Citer

G Gradwohl, A Mazen, G De Murcia. Poly(ADP-ribose) polymerase forms loops with DNA. Biochem Biophys Res Commun, 1987, 148 (3), pp.913-9. ⟨10.1016/s0006-291x(87)80219-x⟩. ⟨hal-02371453⟩

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