Liver ubiquitome uncovers nutrient-stress-mediated trafficking and secretion of complement C3 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cell Death and Disease Année : 2016

Liver ubiquitome uncovers nutrient-stress-mediated trafficking and secretion of complement C3

Résumé

Adaptation to changes in nutrient availability is crucial for cells and organisms. Posttranslational modifications of signaling proteins are very dynamic and are therefore key to promptly respond to nutrient deprivation or overload. Herein we screened for ubiquitylation of proteins in the livers of fasted and refed mice using a comprehensive systemic proteomic approach. Among 1641 identified proteins, 117 were differentially ubiquitylated upon fasting or refeeding. Endoplasmic reticulum (ER) and secretory proteins were enriched in the livers of refed mice in part owing to an ER-stress-mediated response engaging retro-translocation and ubiquitylation of proteins from the ER. Complement C3, an innate immune factor, emerged as the most prominent ER-related hit of our screen. Accordingly, we found that secretion of C3 from the liver and primary hepatocytes as well as its dynamic trafficking are nutrient dependent. Finally, obese mice with a chronic nutrient overload show constitutive trafficking of C3 in the livers despite acute changes in nutrition, which goes in line with increased C3 levels and low-grade inflammation reported for obese patients. Our study thus suggests that nutrient sensing in the liver is coupled to release of C3 and potentially its metabolic and inflammatory functions.
Fichier principal
Vignette du fichier
cddis2016312a.pdf (1.26 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

hal-02371041 , version 1 (24-01-2024)

Identifiants

Citer

Helena de Fatima Magliarelli, Mariette I Matondo, Gergő Mészáros, Alexander Goginashvili, Eric Erbs, et al.. Liver ubiquitome uncovers nutrient-stress-mediated trafficking and secretion of complement C3. Cell Death and Disease, 2016, 7 (10), pp.e2411-e2411. ⟨10.1038/cddis.2016.312⟩. ⟨hal-02371041⟩
36 Consultations
2 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More