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Article Dans Une Revue Biosensors and Bioelectronics Année : 2019

Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins

Résumé

Knowledge on host-pathogen interactions contributes to the development of approaches to alleviate infectious disease. In this work, we developed a surface plasmon resonance (SPR) based method for investigating bacteria/mucins interactions. Furthermore, we investigated adhesion of three pathogens, Aeromonas salmonicida, Aeromonas hydrophila and Vibrio harveyi, to Atlantic salmon mucins isolated from different epithelial sites, using SPR and microtiter-based binding assays. We demonstrated that performing bacterial binding assays to mucins using SPR is feasible and has advantages over microtiter-based binding assays, especially under flow conditions. The fluid flow in the SPR is linear and continuous and SPR enables real-time reading of mucin-bacterial bonds, which provides an in vivo-like setup for analysis of bacterial binding to mucins. The variation between technical replicates was smaller using SPR detection compared to the adenosine 5′-triphosphate (ATP) bioluminescence assay in microtiter plates. Furthermore, we demonstrated that the effect of flow on pathogen-mucin interaction is significant and that bacterial adhesion differ non-linearly with flow rates and depend on the epithelial source of the mucin.
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Dates et versions

hal-02367926 , version 1 (04-02-2020)

Identifiants

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János Tamás Padra, Quentin Pagneux, Julie Bouckaert, Roxana Jijie, Henrik Sundh, et al.. Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins. Biosensors and Bioelectronics, 2019, 146, pp.111736. ⟨10.1016/j.bios.2019.111736⟩. ⟨hal-02367926⟩
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