The control of feed intake in fish in aquaculture requires the development of new techniques to improve dietcomposition, feed conversion efficiency and growth. The aim must be sustainability and an effective use ofresources. The effect of replacing traditional aqua-feed ingredients (fishmeal and fish oil) by a 100% plant-baseddiet is known to drastically decrease fish performance (survival and growth). The present study examined thefeed preference of rainbow trout Oncorhynchus mykiss for three diets containing distinct levels of omega-3 longchain polyunsaturated fatty acids (ω-3 LCPUFA): eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)(0% for low, 5% for medium and 20% for high, total fatty acid content). Feed preference values for each group(low v. medium ω-3 diets, medium v. high ω-3 diets and low v. high ω-3 diets) were observed using two selffeederspositioned at opposite sides of the tank. The hypothesis was that the decrease of fish growth and survivalrate of fish fed with 100% plant-based diet could be explained by the absence of ω-3 LCPUFA relating to decrease of food intake. This could explain the tasting role of ω-3 LCPUFA in the feeding behavior of rainbow trout (which reflects the motivation to consume feed). The results showed that rainbow trout could discriminate between the diets containing different level of ω-3 LCPUFA even if unable to differentiate between level of 5% (no preference observed in low v. medium ω-3 diets). Overall they had a preference for diet high in ω-3 LCPUFA: 59.5% preference for high ω-3 diet in high v. low ω-3 diets, and 75.6% preference for high ω-3 diet in medium v. high ω-3 diets respectively. This preference was repeated after 21 days and for a further 21 days when the feeds were exchanged between the two self- feeders in each tank: 63.3% preference for high ω-3 diet in high v. low ω-3 diets, and 69,5% preference for high ω-3 diet in medium v. high ω-3 diets respectively. The tests also indicated adifference in the extent of food waste of each of the three diets revealed by uneaten pellets after feed demands.During two periods of test, high ω-3 diet was the most appreciated, the least wasted and the most eaten (allchoice groups) whereas the most uneaten feed remained the least appreciated diet in three choices diets (low ω-3 diet in low v. medium ω-3 diets, medium in medium v. high ω-3 diets and low in low v. high ω-3). In conclusion, this study highlighted the influence of ω-3 LCPUFA in the feeding behavior of juvenile rainbow trout, levels of ω-3 LCPUFA drove dietary choices in the fish