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Article Dans Une Revue PLoS Biology Année : 2019

Mechanical stiffness of reconstituted actin patches correlates tightly with endocytosis efficiency

La rigidité de patchs reconstituées de filaments d'actine est fortement corrélée à l'efficacité de l'endocytose

Résumé

Clathrin-mediated endocytosis involves the sequential assembly of more than 60 proteins at the plasma membrane. An important fraction of these proteins regulates the assembly of an actin-related protein 2/3 (Arp2/3)-branched actin network, which is essential to generate the force during membrane invagination. We performed, on wild-type (WT) yeast and mutant strains lacking putative actin crosslinkers, a side-by-side comparison of in vivo endocytic phenotypes and in vitro rigidity measurements of reconstituted actin patches. We found a clear correlation between softer actin networks and a decreased efficiency of endocytosis. Our observations support a chain-of-consequences model in which loss of actin crosslinking softens Arp2/3-branched actin networks, directly limiting the transmission of the force. Additionally , the lifetime of failed endocytic patches increases, leading to a larger number of patches and a reduced pool of polymerizable actin, which slows down actin assembly and further impairs endocytosis.
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hal-02363516 , version 1 (29-11-2019)

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Jessica Planade, Reda Belbahri, Micaela Boiero Sanders, Audrey Guillotin, Olivia Du Roure, et al.. Mechanical stiffness of reconstituted actin patches correlates tightly with endocytosis efficiency. PLoS Biology, 2019, 17 (10), pp.e3000500. ⟨10.1371/journal.pbio.3000500⟩. ⟨hal-02363516⟩
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