<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-02361399</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-24T15:33:26+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Lung deposition and toxicological responses evoked by multi-walled carbon nanotubes dispersed in a synthetic lung surfactant in the mouse</title>
            <author role="aut">
              <persName>
                <forename type="first">Carole</forename>
                <surname>Ronzani</surname>
              </persName>
              <email type="md5">1e1a0b5e888b38345fba4e7fdef72244</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="numeric">1079730</idno>
              <idno type="halauthorid" notation="string">734139-1079730</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-0489-4825</idno>
              <affiliation ref="#struct-207315"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Coralie</forename>
                <surname>Spiegelhalter</surname>
              </persName>
              <email type="md5">5d394cf05c17ca201223d7c62836f6fd</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="numeric">1079732</idno>
              <idno type="halauthorid" notation="string">439956-1079732</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-6372-1647</idno>
              <idno type="IDREF">https://www.idref.fr/250397870</idno>
              <affiliation ref="#struct-1047"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Jean-Luc</forename>
                <surname>Vonesch</surname>
              </persName>
              <email type="md5">e58a22c2f695aca863101b32a894326e</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="numeric">1099679</idno>
              <idno type="halauthorid" notation="string">202058-1099679</idno>
              <affiliation ref="#struct-1047"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Luc</forename>
                <surname>Lebeau</surname>
              </persName>
              <email type="md5">bb7b2970f3b79d9926417aa83f28fe38</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="string">luc-lebeau</idno>
              <idno type="idhal" notation="numeric">739584</idno>
              <idno type="halauthorid" notation="string">8582-739584</idno>
              <idno type="IDREF">https://www.idref.fr/136240445</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-3276-6727</idno>
              <affiliation ref="#struct-207315"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Françoise</forename>
                <surname>Pons</surname>
              </persName>
              <email type="md5">3f3a25ae5d5ad05306caf599f64fb2cc</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="string">francoise-pons</idno>
              <idno type="idhal" notation="numeric">752556</idno>
              <idno type="halauthorid" notation="string">159464-752556</idno>
              <affiliation ref="#struct-207315"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Luc</forename>
                <surname>Lebeau</surname>
              </persName>
              <email type="md5">bb7b2970f3b79d9926417aa83f28fe38</email>
              <email type="domain">unistra.fr</email>
            </editor>
            <funder ref="#projanr-30020"/>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2019-11-13 13:22:50</date>
              <date type="whenModified">2026-02-07 05:17:36</date>
              <date type="whenReleased">2019-11-13 13:22:50</date>
              <date type="whenProduced">2012-01</date>
              <ref type="externalLink" target="https://api.istex.fr/ark:/67375/VQC-99VDJNSD-P/fulltext.pdf?sid=hal"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="809227">
                <persName>
                  <forename>Luc</forename>
                  <surname>Lebeau</surname>
                </persName>
                <email type="md5">bb7b2970f3b79d9926417aa83f28fe38</email>
                <email type="domain">unistra.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-02361399</idno>
            <idno type="halUri">https://hal.science/hal-02361399</idno>
            <idno type="halBibtex">ronzani:hal-02361399</idno>
            <idno type="halRefHtml">&lt;i&gt;Archives of Toxicology&lt;/i&gt;, 2012, 86 (1), pp.137-149. &lt;a target="_blank" href="https://dx.doi.org/10.1007/s00204-011-0741-y"&gt;&amp;#x27E8;10.1007/s00204-011-0741-y&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Archives of Toxicology, 2012, 86 (1), pp.137-149. &amp;#x27E8;10.1007/s00204-011-0741-y&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-STRASBG1">Université Louis Pasteur - Strasbourg I</idno>
            <idno type="stamp" n="IGBMC" corresp="INSERM">Institut Génétique de Biologie Moléculaire et Cellulaire</idno>
            <idno type="stamp" n="UNIV-STRASBG">Université de Strasbourg</idno>
            <idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno>
            <idno type="stamp" n="SITE-ALSACE">Archive ouverte du site Alsace</idno>
            <idno type="stamp" n="ANR">ANR</idno>
            <idno type="stamp" n="TEST2-HALCNRS">TEST2-HALCNRS</idno>
            <idno type="stamp" n="UNIVOAK">UNIVOAK</idno>
            <idno type="stamp" n="CBST">Laboratoire de chémo-biologie synthétique et thérapeutique (CBST)</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Lung deposition and toxicological responses evoked by multi-walled carbon nanotubes dispersed in a synthetic lung surfactant in the mouse</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Carole</forename>
                    <surname>Ronzani</surname>
                  </persName>
                  <email type="md5">1e1a0b5e888b38345fba4e7fdef72244</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="numeric">1079730</idno>
                  <idno type="halauthorid" notation="string">734139-1079730</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-0489-4825</idno>
                  <affiliation ref="#struct-207315"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Coralie</forename>
                    <surname>Spiegelhalter</surname>
                  </persName>
                  <email type="md5">5d394cf05c17ca201223d7c62836f6fd</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="numeric">1079732</idno>
                  <idno type="halauthorid" notation="string">439956-1079732</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-6372-1647</idno>
                  <idno type="IDREF">https://www.idref.fr/250397870</idno>
                  <affiliation ref="#struct-1047"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Jean-Luc</forename>
                    <surname>Vonesch</surname>
                  </persName>
                  <email type="md5">e58a22c2f695aca863101b32a894326e</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="numeric">1099679</idno>
                  <idno type="halauthorid" notation="string">202058-1099679</idno>
                  <affiliation ref="#struct-1047"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Luc</forename>
                    <surname>Lebeau</surname>
                  </persName>
                  <email type="md5">bb7b2970f3b79d9926417aa83f28fe38</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="string">luc-lebeau</idno>
                  <idno type="idhal" notation="numeric">739584</idno>
                  <idno type="halauthorid" notation="string">8582-739584</idno>
                  <idno type="IDREF">https://www.idref.fr/136240445</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-3276-6727</idno>
                  <affiliation ref="#struct-207315"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Françoise</forename>
                    <surname>Pons</surname>
                  </persName>
                  <email type="md5">3f3a25ae5d5ad05306caf599f64fb2cc</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="string">francoise-pons</idno>
                  <idno type="idhal" notation="numeric">752556</idno>
                  <idno type="halauthorid" notation="string">159464-752556</idno>
                  <affiliation ref="#struct-207315"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">10731</idno>
                <idno type="issn">0340-5761</idno>
                <idno type="eissn">1432-0738</idno>
                <title level="j">Archives of Toxicology</title>
                <imprint>
                  <publisher>Springer Verlag</publisher>
                  <biblScope unit="volume">86</biblScope>
                  <biblScope unit="issue">1</biblScope>
                  <biblScope unit="pp">137-149</biblScope>
                  <date type="datePub">2012-01</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1007/s00204-011-0741-y</idno>
              <idno type="pubmed">21805258</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="chim">Chemical Sciences</classCode>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>In the present work, we elaborated a synthetic lung surfactant composed of dipalmitoyl phosphatidylcholine (DPPC), phosphatidylglycerol, cholesterol and bovine serum albumin (BSA), as a vehicle to study the lung toxicity of pristine multi-walled carbon nanotubes (MWCNT). MWCNT were dispersed in surfactant, saline or saline containing DPPC, BSA, Pluronic(®) F68 or sodium dodecyl sulfate, for comparison. Dispersions were characterized visually, and by light microscopy, dynamic light scattering and transmission electronic microscopy (TEM). Deposition of surfactant-dispersed MWCNT in the lung of BALB/c mice upon single or repeated administrations was analyzed by histology and TEM. Inflammation and airway remodeling were assessed in bronchoalveolar lavage fluid (BALF) or lung tissue of mice by counting cells and quantifying cytokines, tumor growth factor (TGF)-β1 and collagen, and by histology. We found that the elaborated surfactant is more effective in dispersing MWCNT when compared to the other agents, while being biocompatible. Surfactant-dispersed MWCNT distributed all throughout the mouse airways upon single and repeated administrations and were observed in alveolar macrophages and epithelial cells, and in infiltrated neutrophils. Mice that received a single administration of MWCNT showed neutrophil infiltrate and greater concentrations of tumor necrosis factor (TNF)-α, keratinocyte-derived chemokine (KC) and interleukin (IL)-17 in BALF when compared to controls. After repeated MWCNT administrations, increases in macrophage number, KC and TGF-β1 levels in BALF, and collagen deposition and mucus hyperplasia in lung tissue were observed. Altogether, the elaborated lung surfactant could be a valuable tool to further study the toxicological impact of pristine MWCNT in laboratory animals.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-207315" status="OLD">
          <idno type="ISNI">0000000403871645</idno>
          <idno type="RNSR">200918483F</idno>
          <idno type="ROR">https://ror.org/00rh71z37</idno>
          <orgName>Conception et application de molécules bioactives</orgName>
          <orgName type="acronym">CAMB</orgName>
          <date type="start">2009-01-01</date>
          <date type="end">2023-12-31</date>
          <desc>
            <address>
              <addrLine>Faculté de Pharmacie 74 route du Rhin BP 60024 67401 Illkirch Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.camb.cnrs.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-199013" type="direct"/>
            <relation active="#struct-341038" type="direct"/>
            <relation name="UMR7199" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-1047" status="OLD">
          <orgName>Institut de génétique et biologie moléculaire et cellulaire</orgName>
          <orgName type="acronym">IGBMC</orgName>
          <date type="end">2008-12-31</date>
          <desc>
            <address>
              <addrLine>I.G.B.M.C, Parc D'Innovation 1 Rue Laurent Fries - BP 10142 67404 ILLKIRCH CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www-ulp.u-strasbg.fr/unite_recherche.php?u=14</ref>
          </desc>
          <listRelation>
            <relation active="#struct-300320" type="direct"/>
            <relation name="U596" active="#struct-303623" type="direct"/>
            <relation name="UMR7104" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-199013" status="VALID">
          <idno type="IdRef">131056549</idno>
          <idno type="ROR">https://ror.org/00pg6eq24</idno>
          <orgName>Université de Strasbourg</orgName>
          <orgName type="acronym">UNISTRA</orgName>
          <desc>
            <address>
              <addrLine>4 rue Blaise Pascal - CS 90032 - 67081 Strasbourg cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.unistra.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-341038" status="VALID">
          <idno type="IdRef">232435103</idno>
          <idno type="ISNI">000000010626358X</idno>
          <idno type="ROR">https://ror.org/02cte4b68</idno>
          <orgName>Institut de Chimie - CNRS Chimie</orgName>
          <orgName type="acronym">INC-CNRS</orgName>
          <date type="start">2009-10-01</date>
          <desc>
            <address>
              <addrLine>CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE3 rue Michel-Ange75794 PARIS CEDEX 16 - France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cnrs.fr/inc</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-300320" status="OLD">
          <orgName>Université Louis Pasteur - Strasbourg I</orgName>
          <date type="start">1970-01-01</date>
          <date type="end">2009-01-01</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="anrProject" xml:id="projanr-30020" status="VALID">
          <idno type="anr">ANR-08-CESA-0017</idno>
          <idno type="program">Contaminants Ecosystèmes et Santé</idno>
          <orgName>INCAA</orgName>
          <desc>Impact de l'exposition aux nanotubes de carbone sur la survenue et la sévérité de l'asthme allergique</desc>
          <date type="start">2008</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>