Performance of the automated multiplex PCR Unyvero implant and tissue infections system in the management of diabetic foot osteomyelitis
Résumé
AIM: We evaluated the performance of Unyvero implant and tissue infections system (ITI) application (Curetis) to diagnose Diabetic Foot Osteomyelitis (DFOM). PATIENTS & METHODS: The study was conducted in the Diabetic Foot reference center of N\ⁱmes University Hospital (France) from 1 December 2016 to 31 May 2017. We compared the Unyvero ITI PCR to conventional culture and alternative molecular approaches. RESULTS: A total of 79 patients with DFOM were included: 177 microorganisms were isolated by culture, 146 detected by PCR, resulting in a concordance level of 66.7% (65.0-68.4). Discrepant results were obtained for 45 samples, with 59 microorganisms being detected by PCR only (18 samples) or by culture only (27 samples). CONCLUSION: Unyvero ITI PCR represents an interesting additional diagnosis solution to manage DFOM.
Mots clés
Humans
Female
Male
Adult
Aged
Middle Aged
80 and over
France
Multiplex Polymerase Chain Reaction
resistance
Disease Management
Diabetes Mellitus
Type 1
Sepsis
Type 2
Bacteria
culture
Diabetic Foot
diabetic foot osteomyelitis
Drug Resistance
Microbial
Fungi
microbial diagnosis
multiplex PCR
Osteomyelitis
Prostheses and Implants
Prosthesis-Related Infections
Unyvero ITI