<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-02357456</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-24T19:08:00+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Profiling of derived-hepatocyte progenitors from induced pluripotent stem cells using nanoCAGE promoter analysis</title>
            <author role="aut">
              <persName>
                <forename type="first">Myriam</forename>
                <surname>Lereau Bernier</surname>
              </persName>
              <idno type="halauthorid">1708210-0</idno>
              <affiliation ref="#struct-2208"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Stéphane</forename>
                <surname>Poulain</surname>
              </persName>
              <idno type="idhal" notation="numeric">760732</idno>
              <idno type="halauthorid" notation="string">372464-760732</idno>
              <idno type="IDREF">https://www.idref.fr/135531853</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-5503-1723</idno>
              <affiliation ref="#struct-455818"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Yannick</forename>
                <forename type="middle">Y.</forename>
                <surname>Tauran</surname>
              </persName>
              <email type="md5">6d88aac29e12788128ffb503acdcd53b</email>
              <email type="domain">univ-lyon1.fr</email>
              <idno type="idhal" notation="string">yannick-tauran</idno>
              <idno type="idhal" notation="numeric">179546</idno>
              <idno type="halauthorid" notation="string">837900-179546</idno>
              <idno type="IDREF">https://www.idref.fr/183440919</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-0706-7799</idno>
              <affiliation ref="#struct-752"/>
              <affiliation ref="#struct-2208"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Mathieu</forename>
                <surname>Danoy</surname>
              </persName>
              <idno type="halauthorid">827578-0</idno>
              <affiliation ref="#struct-2208"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Marie</forename>
                <surname>Shinohara</surname>
              </persName>
              <idno type="halauthorid">1568668-0</idno>
              <affiliation ref="#struct-1004295"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Keiichi</forename>
                <surname>Kimura</surname>
              </persName>
              <idno type="halauthorid">1568669-0</idno>
              <affiliation ref="#struct-1004295"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Bertrand David</forename>
                <surname>Segard</surname>
              </persName>
              <idno type="halauthorid">1201608-0</idno>
              <affiliation ref="#struct-2208"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sachi</forename>
                <surname>Kato</surname>
              </persName>
              <idno type="halauthorid">1065154-0</idno>
              <affiliation ref="#struct-455818"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Taketomo</forename>
                <surname>Kido</surname>
              </persName>
              <idno type="halauthorid">1568670-0</idno>
              <affiliation ref="#struct-89801"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Atsushi</forename>
                <surname>Miyajima</surname>
              </persName>
              <idno type="halauthorid">1568671-0</idno>
              <affiliation ref="#struct-89801"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Yasuyuki</forename>
                <surname>Sakai</surname>
              </persName>
              <idno type="halauthorid">812138-0</idno>
              <affiliation ref="#struct-2208"/>
              <affiliation ref="#struct-1004295"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Charles</forename>
                <surname>Plessy</surname>
              </persName>
              <idno type="halauthorid">241281-0</idno>
              <affiliation ref="#struct-455818"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Eric</forename>
                <surname>Leclerc</surname>
              </persName>
              <email type="md5">77ac44586e76c4e1284ee40c042940e5</email>
              <email type="domain">utc.fr</email>
              <idno type="idhal" notation="string">cnrslec</idno>
              <idno type="idhal" notation="numeric">945746</idno>
              <idno type="halauthorid" notation="string">12957-945746</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-7397-6205</idno>
              <affiliation ref="#struct-2208"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Eric</forename>
                <surname>Leclerc</surname>
              </persName>
              <email type="md5">77ac44586e76c4e1284ee40c042940e5</email>
              <email type="domain">utc.fr</email>
            </editor>
            <funder ref="#projanr-42442"/>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2019-11-10 10:55:20</date>
              <date type="whenModified">2026-02-02 14:42:04</date>
              <date type="whenReleased">2019-11-10 10:55:20</date>
              <date type="whenProduced">2019-02</date>
              <ref type="externalLink" target="https://api.istex.fr/ark:/67375/6H6-6G5R79JX-P/fulltext.pdf?sid=hal"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="809833">
                <persName>
                  <forename>Eric</forename>
                  <surname>Leclerc</surname>
                </persName>
                <email type="md5">77ac44586e76c4e1284ee40c042940e5</email>
                <email type="domain">utc.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-02357456</idno>
            <idno type="halUri">https://hal.science/hal-02357456</idno>
            <idno type="halBibtex">lereaubernier:hal-02357456</idno>
            <idno type="halRefHtml">&lt;i&gt;Biochemical Engineering Journal&lt;/i&gt;, 2019, 142, pp.7-17. &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.bej.2018.11.001"&gt;&amp;#x27E8;10.1016/j.bej.2018.11.001&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Biochemical Engineering Journal, 2019, 142, pp.7-17. &amp;#x27E8;10.1016/j.bej.2018.11.001&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-LYON1">Université Claude Bernard - Lyon I</idno>
            <idno type="stamp" n="LMI" corresp="UNIV-LYON1">Laboratoire des Multimatériaux et Interfaces</idno>
            <idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno>
            <idno type="stamp" n="LIMMS">Laboratory for Integrated Micro Mechatronics Systems</idno>
            <idno type="stamp" n="UDL">UDL</idno>
            <idno type="stamp" n="UNIV-LYON">Université de Lyon</idno>
            <idno type="stamp" n="TEST-HALCNRS">Collection test HAL CNRS</idno>
            <idno type="stamp" n="ANR">ANR</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Profiling of derived-hepatocyte progenitors from induced pluripotent stem cells using nanoCAGE promoter analysis</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Myriam</forename>
                    <surname>Lereau Bernier</surname>
                  </persName>
                  <idno type="halauthorid">1708210-0</idno>
                  <affiliation ref="#struct-2208"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Stéphane</forename>
                    <surname>Poulain</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">760732</idno>
                  <idno type="halauthorid" notation="string">372464-760732</idno>
                  <idno type="IDREF">https://www.idref.fr/135531853</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-5503-1723</idno>
                  <affiliation ref="#struct-455818"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Yannick</forename>
                    <forename type="middle">Y.</forename>
                    <surname>Tauran</surname>
                  </persName>
                  <email type="md5">6d88aac29e12788128ffb503acdcd53b</email>
                  <email type="domain">univ-lyon1.fr</email>
                  <idno type="idhal" notation="string">yannick-tauran</idno>
                  <idno type="idhal" notation="numeric">179546</idno>
                  <idno type="halauthorid" notation="string">837900-179546</idno>
                  <idno type="IDREF">https://www.idref.fr/183440919</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-0706-7799</idno>
                  <affiliation ref="#struct-752"/>
                  <affiliation ref="#struct-2208"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Mathieu</forename>
                    <surname>Danoy</surname>
                  </persName>
                  <idno type="halauthorid">827578-0</idno>
                  <affiliation ref="#struct-2208"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Marie</forename>
                    <surname>Shinohara</surname>
                  </persName>
                  <idno type="halauthorid">1568668-0</idno>
                  <affiliation ref="#struct-1004295"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Keiichi</forename>
                    <surname>Kimura</surname>
                  </persName>
                  <idno type="halauthorid">1568669-0</idno>
                  <affiliation ref="#struct-1004295"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Bertrand David</forename>
                    <surname>Segard</surname>
                  </persName>
                  <idno type="halauthorid">1201608-0</idno>
                  <affiliation ref="#struct-2208"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sachi</forename>
                    <surname>Kato</surname>
                  </persName>
                  <idno type="halauthorid">1065154-0</idno>
                  <affiliation ref="#struct-455818"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Taketomo</forename>
                    <surname>Kido</surname>
                  </persName>
                  <idno type="halauthorid">1568670-0</idno>
                  <affiliation ref="#struct-89801"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Atsushi</forename>
                    <surname>Miyajima</surname>
                  </persName>
                  <idno type="halauthorid">1568671-0</idno>
                  <affiliation ref="#struct-89801"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Yasuyuki</forename>
                    <surname>Sakai</surname>
                  </persName>
                  <idno type="halauthorid">812138-0</idno>
                  <affiliation ref="#struct-2208"/>
                  <affiliation ref="#struct-1004295"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Charles</forename>
                    <surname>Plessy</surname>
                  </persName>
                  <idno type="halauthorid">241281-0</idno>
                  <affiliation ref="#struct-455818"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Eric</forename>
                    <surname>Leclerc</surname>
                  </persName>
                  <email type="md5">77ac44586e76c4e1284ee40c042940e5</email>
                  <email type="domain">utc.fr</email>
                  <idno type="idhal" notation="string">cnrslec</idno>
                  <idno type="idhal" notation="numeric">945746</idno>
                  <idno type="halauthorid" notation="string">12957-945746</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-7397-6205</idno>
                  <affiliation ref="#struct-2208"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">11099</idno>
                <idno type="issn">1369-703X</idno>
                <title level="j">Biochemical Engineering Journal</title>
                <imprint>
                  <publisher>Elsevier</publisher>
                  <biblScope unit="volume">142</biblScope>
                  <biblScope unit="pp">7-17</biblScope>
                  <date type="datePub">2019-02</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1016/j.bej.2018.11.001</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">hiPS</term>
                <term xml:lang="en">Hepatic differentiation</term>
                <term xml:lang="en">nanoCAGE</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Human induced pluripotent stem (hiPS) cells represent a potential source of human cells for regenerative and personalized medicine applications. It is envisioned that hiPS cells could be an efficient tool for disease modelling and drug therapy testings. However, the successful derivation of hiPS cells to mature hepatocytes remains a challenge as far as the current proposed protocols lead to immature patterns and are very expensive (due to growth factors cost). In this work, we have compared two protocols of differentiation to produce hepatocyte-like cells. The first protocol is the conventional protocol adapted from Si-Tayeb et al. in 2010. By changing the frequency of culture medium exchange, we reduced the use of growth factors in the second protocol by 40%. Both protocols lead to hepatocyte-like characteristics including albumin (ALB) and cytochrome P450/3A4 (CYP3A4) expression in cells. Albumin production reached 2500 ng/mL/day for both protocols. The transcription factor motif activity analysis via transcriptomic data obtained with the Cap Analysis Gene Expression (CAGE) technology confirmed a typical hepatic profile for both protocols (such as activation of HNF1 A and HNF1B promoters). However, specific patterns were also detected with higher mRNA levels of WT1, MTF1, HCFC1, and PGCα1 in protocol 2. The overall transcriptomic analysis also revealed a modulation of targets involved in glucose homeostasis, in cell remodeling, lipid pathways and chromatin processing. However, the alpha-fetoprotein (AFP) production remained very high and the function of CYP3A4 was weak in both protocols illustrating still an immature profile in both protocols.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-2208" status="VALID">
          <idno type="RNSR">200419757M</idno>
          <idno type="ROR">0059vr703</idno>
          <orgName>Laboratory for Integrated Micro Mechatronics Systems</orgName>
          <orgName type="acronym">LIMMS</orgName>
          <desc>
            <address>
              <addrLine>LIMMS/CNRS-IIS(UMI 2820)/ Institute of Industrial Science, University of Tokyo/ 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505 Japan/</addrLine>
              <country key="JP"/>
            </address>
            <ref type="url">http://www.fujita3.iis.u-tokyo.ac.jp/~limms</ref>
          </desc>
          <listRelation>
            <relation active="#struct-304304" type="direct"/>
            <relation name="IRL2820 / UMI2820" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-455818" status="VALID">
          <orgName>RIKEN Center for Life Science Technologies</orgName>
          <orgName type="acronym">RIKEN CLST</orgName>
          <desc>
            <address>
              <country key="JP"/>
            </address>
            <ref type="url">http://www.clst.riken.jp/en/#</ref>
          </desc>
          <listRelation>
            <relation active="#struct-26075" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-752" status="VALID">
          <idno type="IdRef">151655073</idno>
          <idno type="ISNI">0000000403846669</idno>
          <idno type="RNSR">199512045U</idno>
          <idno type="ROR">https://ror.org/03cfem402</idno>
          <orgName>Laboratoire des Multimatériaux et Interfaces</orgName>
          <orgName type="acronym">LMI</orgName>
          <date type="start">1995-01-01</date>
          <desc>
            <address>
              <addrLine>bat. 731 43 Bvd du 11 Novembre 1918 69622 VILLEURBANNE CEDEX, puis à partir de juillet 2017 : Bâtiment Chevreul 6 rue Victor Grignard 69622 Villeurbanne CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://lmi.cnrs.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-194495" type="direct"/>
            <relation active="#struct-301088" type="indirect"/>
            <relation active="#struct-341038" type="direct"/>
            <relation name="UMR5615" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-1004295" status="VALID">
          <orgName>Center for International Research on Integrative Biomedical Systems [University of Tokyo]</orgName>
          <orgName type="acronym">CIBiS</orgName>
          <desc>
            <address>
              <addrLine>Institute of Industrial Science, the University of Tokyo</addrLine>
              <country key="JP"/>
            </address>
            <ref type="url">http://www.cibis.iis.u-tokyo.ac.jp/en/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-50647" type="direct"/>
            <relation active="#struct-304304" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-89801" status="INCOMING">
          <orgName>Institute of Molecular and Cellular Biosciences</orgName>
          <desc>
            <address>
              <addrLine>Tokyo 113-0032</addrLine>
              <country key="JP"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-304304" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-304304" status="VALID">
          <idno type="IdRef">029909848</idno>
          <idno type="ROR">https://ror.org/057zh3y96</idno>
          <orgName>The University of Tokyo</orgName>
          <orgName type="acronym">UTokyo</orgName>
          <desc>
            <address>
              <addrLine>7 Chome-3-1 Hongo, Bunkyo, Tokyo 113-8654</addrLine>
              <country key="JP"/>
            </address>
            <ref type="url">https://www.u-tokyo.ac.jp/en/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-26075" status="VALID">
          <idno type="ISNI">0000 0000 9446 5255</idno>
          <idno type="ROR">https://ror.org/01sjwvz98</idno>
          <idno type="Wikidata">Q1153275</idno>
          <orgName>RIKEN - Institute of Physical and Chemical Research [Japon]</orgName>
          <orgName type="acronym">RIKEN</orgName>
          <date type="start">1917-03-20</date>
          <desc>
            <address>
              <addrLine>Hirosawa 2-1, Wako, Saitama 351-0198</addrLine>
              <country key="JP"/>
            </address>
            <ref type="url">https://www.riken.jp/en/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-194495" status="VALID">
          <idno type="IdRef">026402823</idno>
          <idno type="ISNI">0000000121686185</idno>
          <idno type="ROR">https://ror.org/029brtt94</idno>
          <orgName>Université Claude Bernard Lyon 1</orgName>
          <orgName type="acronym">UCBL</orgName>
          <desc>
            <address>
              <addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-lyon1.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-301088" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-301088" status="VALID">
          <idno type="ROR">https://ror.org/01rk35k63</idno>
          <orgName>Université de Lyon</orgName>
          <desc>
            <address>
              <addrLine>92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.universite-lyon.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-341038" status="VALID">
          <idno type="IdRef">232435103</idno>
          <idno type="ISNI">000000010626358X</idno>
          <idno type="ROR">https://ror.org/02cte4b68</idno>
          <orgName>Institut de Chimie - CNRS Chimie</orgName>
          <orgName type="acronym">INC-CNRS</orgName>
          <date type="start">2009-10-01</date>
          <desc>
            <address>
              <addrLine>CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE3 rue Michel-Ange75794 PARIS CEDEX 16 - France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cnrs.fr/inc</ref>
          </desc>
        </org>
        <org type="regrouplaboratory" xml:id="struct-50647" status="VALID">
          <orgName>Institute of Industrial Science [Tokyo]</orgName>
          <orgName type="acronym">IIS</orgName>
          <desc>
            <address>
              <addrLine>4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan</addrLine>
              <country key="JP"/>
            </address>
            <ref type="url">http://dfs.iis.u-tokyo.ac.jp/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-304304" type="direct"/>
          </listRelation>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="anrProject" xml:id="projanr-42442" status="VALID">
          <idno type="anr">ANR-16-RHUS-0005</idno>
          <orgName>iLite</orgName>
          <desc>iLite</desc>
          <date type="start">2016</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>