Quantification and Characterization of UVB-Induced Mitochondrial Fragmentation in Normal Primary Human Keratinocytes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2016

Quantification and Characterization of UVB-Induced Mitochondrial Fragmentation in Normal Primary Human Keratinocytes

Romain Jugé
  • Fonction : Auteur
Josselin Breugnot
  • Fonction : Auteur
  • PersonId : 766132
  • IdRef : 160629136
Célia da Silva
  • Fonction : Auteur
Sylvie Bordes
  • Fonction : Auteur
Brigitte Closs
  • Fonction : Auteur

Résumé

UV irradiation is a major environmental factor causing skin dryness, aging and cancer. UVB in particular triggers cumulative DNA damage, oxidative stress and mitochondrial dysfunction. The objective of our study was to provide both qualitative and quantitative analysis of how mitochondria respond to UVB irradiation in normal human epidermal keratinocytes (NHEK) of healthy donors, with the rationale that monitoring mitochondrial shape will give an indication of cell population fitness and enable the screening of bioactive agents with UVB-protective properties. Our results show that NHEK undergo dose-dependent mitochondrial fragmentation after exposure to UVB. In order to obtain a quantitative measure of this phenomenon, we implemented a novel tool for automated quantification of mitochondrial morphology in live cells based on confocal microscopy and computational calculations of mitochondrial shape descriptors. This method was used to substantiate the effects on mitochondrial morphology of UVB irradiation and of knocking-down the mitochondrial fission-mediating GTPase Dynamin-related protein 1 (DRP1). Our data further indicate that all the major mitochondrial dynamic proteins are expressed in NHEK but that their level changes were stronger after mitochondrial uncoupler treatment than following UVB irradiation or DRP1 knock-down. Our system and procedures might be of interest for the identification of cosmetic or dermatologic UVB-protective agents.
Fichier principal
Vignette du fichier
srep35065.pdf (1.43 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02355709 , version 1 (08-11-2019)

Licence

Identifiants

Citer

Romain Jugé, Josselin Breugnot, Célia da Silva, Sylvie Bordes, Brigitte Closs, et al.. Quantification and Characterization of UVB-Induced Mitochondrial Fragmentation in Normal Primary Human Keratinocytes. Scientific Reports, 2016, 6 (1), ⟨10.1038/srep35065⟩. ⟨hal-02355709⟩
97 Consultations
66 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More