Biochemical reconstitution and characterization of multicomponent drug efflux transporters - Archive ouverte HAL
Special Issue Methods in Molecular Biology Year : 2018

Biochemical reconstitution and characterization of multicomponent drug efflux transporters

Abstract

Efflux pumps are the major determinants in bacterial multidrug resistance. In Gram-negative bacteria, efflux transporters are organized as macromolecular tripartite machineries that span the two-membrane cell envelope of the bacterium. Biochemical data on purified proteins are essential to draw a mechanistic picture of this highly dynamical, multicomponent, efflux system. We describe protocols for the reconstitution and the in vitro study of transporters belonging to RND and ABC superfamilies: the AcrAB–TolC and MacAB–TolC efflux systems from Escherichia coli and the MexAB–OprM efflux pump from $Pseudomonas\ aeruginosa$.
Fichier principal
Vignette du fichier
Picard-MMB17-transport-2018.pdf (604.23 Ko) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-02350093 , version 1 (28-05-2024)

Identifiers

Cite

Martin Picard, Elena Tikhonova, Isabelle Broutin, Shuo Lu, Alice Verchère, et al.. Biochemical reconstitution and characterization of multicomponent drug efflux transporters. Methods in Molecular Biology, 1700, pp.113-145, 2018, Bacterial Multidrug Exporters Methods and Protocols, ⟨10.1007/978-1-4939-7454-2_8⟩. ⟨hal-02350093⟩
35 View
15 Download

Altmetric

Share

More