N°Spécial De Revue/Special Issue Methods in Molecular Biology Année : 2018

Biochemical reconstitution and characterization of multicomponent drug efflux transporters

Résumé

Efflux pumps are the major determinants in bacterial multidrug resistance. In Gram-negative bacteria, efflux transporters are organized as macromolecular tripartite machineries that span the two-membrane cell envelope of the bacterium. Biochemical data on purified proteins are essential to draw a mechanistic picture of this highly dynamical, multicomponent, efflux system. We describe protocols for the reconstitution and the in vitro study of transporters belonging to RND and ABC superfamilies: the AcrAB–TolC and MacAB–TolC efflux systems from Escherichia coli and the MexAB–OprM efflux pump from $Pseudomonas\ aeruginosa$.

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hal-02350093 , version 1 (28-05-2024)

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Martin Picard, Elena Tikhonova, Isabelle Broutin, Shuo Lu, Alice Verchère, et al.. Biochemical reconstitution and characterization of multicomponent drug efflux transporters. Methods in Molecular Biology, 1700, pp.113-145, 2018, Bacterial Multidrug Exporters Methods and Protocols, ⟨10.1007/978-1-4939-7454-2_8⟩. ⟨hal-02350093⟩
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