Biochemical reconstitution and characterization of multicomponent drug efflux transporters
Abstract
Efflux pumps are the major determinants in bacterial multidrug resistance. In Gram-negative bacteria,
efflux transporters are organized as macromolecular tripartite machineries that span the two-membrane cell
envelope of the bacterium. Biochemical data on purified proteins are essential to draw a mechanistic picture
of this highly dynamical, multicomponent, efflux system. We describe protocols for the reconstitution and
the in vitro study of transporters belonging to RND and ABC superfamilies: the AcrAB–TolC and
MacAB–TolC efflux systems from Escherichia coli and the MexAB–OprM efflux pump from $Pseudomonas\
aeruginosa$.
Domains
Life Sciences [q-bio]Origin | Files produced by the author(s) |
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