A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation - Archive ouverte HAL Access content directly
Journal Articles Nature Communications Year : 2019

A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

Haseeb Qureshi
  • Function : Author
Lucy Wheatley
  • Function : Author
Ledean Cooper
  • Function : Author
Aleksandra Jasiulewicz
  • Function : Author
Huy van Nguyen
  • Function : Author
Joseph Wragg
  • Function : Author
Divyasree Poovathumkadavil
  • Function : Author
Sarah Bajan
  • Function : Author
Attila Sik
  • Function : Author
György Hutvàgner
  • Function : Author
Agnieszka Gambus
  • Function : Author
John Fossey
  • Function : Author
Ferenc Müller
  • Function : Author

Abstract

Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals tran-scriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.
Fichier principal
Vignette du fichier
s41467-019-08487-5.pdf (4.11 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02349535 , version 1 (09-11-2020)

Identifiers

Cite

Yavor Hadzhiev, Haseeb Qureshi, Lucy Wheatley, Ledean Cooper, Aleksandra Jasiulewicz, et al.. A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation. Nature Communications, 2019, 10 (1), ⟨10.1038/s41467-019-08487-5⟩. ⟨hal-02349535⟩
62 View
32 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More