Fast quantitative 2D NMR for metabolomics and lipidomics: A tutorial
Résumé
Nuclear Magnetic Resonance (NMR) has a great potential for the quantitative analysis of complex biological samples. The acquisition of 2D NMR spectra allows a better separation between overlapped resonances while yielding accurate quantitative data when appropriate analytical protocols are implemented. The experiment duration can be made compatible with high-throughput studies by relying on fast acquisition methods. This tutorial describes the general workflow to acquire fast quantitative 2D NMR data for metabolomics and lipidomics applications.
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