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Article Dans Une Revue Electrophoresis Année : 2003

A planar microfabricated nanoelectrospray emitter tip based on a capillary slot

Résumé

We report on the fabrication and testing of planar nib-like structures for nanoelectrospray ionization-mass spectrometry (nanoESI-MS) applications. The micro-nib structures were fabricated on silicon substrates using the negative photoresist SU-8; they include capillary slots with widths of 8 and 16 μm. A suitable wafer cleaving step made the nib-like structures overhang the edge of a silicon substrate to provide a robust interface for nanoESI-MS applications; this freeing of the nib tip from the wafer surface created a point-like structure that is essential to establish an electrospray. The micro-nib sources were successfully tested on an LCQ Deca XP+ ion trap mass spectrometer using peptide samples at concentrations down to 1 μm. The high voltage was applied using a platinum wire inserted in the sample reservoir upstream to the capillary slot. A Taylor cone was clearly seen at the nib tip. The micro-nibs performed well at voltages as low as 0.8 kV; such performances are state-of-the-art with respect to current micromachined ESI-MS interfaces and are conditions comparable to those used for standard emitter tips. In addition, we clearly observed the influence of the micro-nib slot width on the ionization performances: the narrower the slot, the better the performances.

Dates et versions

hal-02347822 , version 1 (05-11-2019)

Identifiants

Citer

Séverine Le Gac, S. Arscott, Christian Rolando. A planar microfabricated nanoelectrospray emitter tip based on a capillary slot. Electrophoresis, 2003, 24 (21), pp.3640-3647. ⟨10.1002/elps.200305664⟩. ⟨hal-02347822⟩
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