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Article Dans Une Revue Journal of Chromatography A Année : 2005

Integrated microfabricated systems including a purification module and an on-chip nano electrospray ionization interface for biological analysis

Résumé

We report here on an integrated microfabricated device dedicated to the preparation of biological samples prior to their on-line analysis by electrospray ionization-mass spectrometry (ESI-MS). This microfluidic device is fabricated using the negative photoresist SU-8 by microtechnology techniques. The device includes a chromatographic module plus an ESI interface for MS. The chromatographic module is dedicated to sample purification and is based on a polymer monolithic phase which includes hydrophobic moieties. The ESI interface is integrated onto the chip and is based on a capillary slot. We present here the integration of these different modules onto a single system that is fabricated via a SU-8-based microtechnology route. We present also their testing for the purification of peptide samples. This started with a partial integration step with the combination of at least two of the modules (microsystem + monolith; microsystem + nib) and their test before the fabrication and testing of fully integrated microsystems.

Dates et versions

hal-02347625 , version 1 (05-11-2019)

Identifiants

Citer

Julien Carlier, S. Arscott, V. Thomy, Jean-Christophe Camart, Cécile Cren-Olivé, et al.. Integrated microfabricated systems including a purification module and an on-chip nano electrospray ionization interface for biological analysis. Journal of Chromatography A, 2005, 1071 (1-2), pp.213-222. ⟨10.1016/j.chroma.2004.12.037⟩. ⟨hal-02347625⟩
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