Article Dans Une Revue Journal of Biological Chemistry Année : 2006

Deficiency in Phylloquinone (Vitamin K 1 ) Methylation Affects Prenyl Quinone Distribution, Photosystem I Abundance, and Anthocyanin Accumulation in the Arabidopsis AtmenG Mutant

Résumé

Phylloquinone (vitamin K 1) is synthesized in cyanobacteria and in chloroplasts of plants, where it serves as electron carrier of photosystem I. The last step of phylloquinone synthesis in cyanobacteria is the methylation of 2-phytyl-1,4-naphthoqui-none by the menG gene product. Here, we report that the uncharacterized Arabidopsis gene At1g23360, which shows sequence similarity to menG, functionally complements the Synechocystis menG mutant. An Arabidopsis mutant, AtmenG, carrying a T-DNA insertion in the gene At1g23360 is devoid of phylloquinone, but contains an increased amount of 2-phytyl-1,4-naphthoquinone. Phylloquinone and 2-phytyl-1,4-naph-thoquinone in thylakoid membranes of wild type and AtmenG, respectively, predominantly localize to photosystem I, whereas excess amounts of prenyl quinones are stored in plastoglobules. Photosystem I reaction centers are decreased in AtmenG plants under high light, as revealed by immunoblot and spectroscopic measurements. Anthocyanin accumulation and chalcone syn-thase (CHS1) transcription are affected during high light exposure , indicating that alterations in photosynthesis in AtmenG affect gene expression in the nucleus. Photosystem II quantum yield is decreased under high light. Therefore, the loss of phyl-loquinone methylation affects photosystem I stability or turnover , and the limitation in functional photosystem I complexes results in overreduction of photosystem II under high light.

Fichier principal
Vignette du fichier
lohmann2006JBC.pdf (608 Ko) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Licence
Loading...

Dates et versions

hal-02347280 , version 1 (05-11-2019)

Licence

Identifiants

Citer

Antje Lohmann, Mark Aurel Schöttler, Claire Bréhélin, Felix Kessler, Ralph Bock, et al.. Deficiency in Phylloquinone (Vitamin K 1 ) Methylation Affects Prenyl Quinone Distribution, Photosystem I Abundance, and Anthocyanin Accumulation in the Arabidopsis AtmenG Mutant. Journal of Biological Chemistry, 2006, 281 (52), pp.40461-40472. ⟨10.1074/jbc.M609412200⟩. ⟨hal-02347280⟩
130 Consultations
142 Téléchargements

Altmetric

Partager

  • More