A molecular dynamics study of adenylyl cyclase: The impact of ATP and G-protein binding - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2018

A molecular dynamics study of adenylyl cyclase: The impact of ATP and G-protein binding

Résumé

Adenylyl cyclases (ACs) catalyze the biosynthesis of cyclic adenosine monophosphate (cAMP) from adenosine triphosphate (ATP) and play an important role in many signal transduction pathways. The enzymatic activity of ACs is carefully controlled by a variety of molecules, including G-protein subunits that can both stimulate and inhibit cAMP production. Using homology models developed from existing structural data, we have carried out all-atom, microsecond-scale molecular dynamics simulations on the AC5 isoform of adenylyl cyclase and on its complexes with ATP and with the stimulatory G-protein subunit Gsα. The results show that both ATP and Gsα binding have significant effects on the structure and flexibility of adenylyl cyclase. New data on ATP bound to AC5 in the absence of Gsα notably help to explain how Gsα binding enhances enzyme activity and could aid product release. Simulations also suggest a possible coupling between ATP binding and interactions with the inhibitory G-protein subunit Gαi
Fichier principal
Vignette du fichier
8a59aca7ddb934efb917691e8bb64674.pdf (8.14 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02344727 , version 1 (28-02-2024)

Identifiants

Citer

Elisa Frezza, Juliette Martin, Richard Lavery. A molecular dynamics study of adenylyl cyclase: The impact of ATP and G-protein binding. PLoS ONE, 2018, 13 (4), pp.e0196207. ⟨10.1371/journal.pone.0196207⟩. ⟨hal-02344727⟩
17 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More