The elastin peptides-mediated induction of pro-collagenase-1 production by human fibroblasts involves activation of MEK/ERK pathway via PKA- and PI 3 K-dependent signaling - Archive ouverte HAL
Article Dans Une Revue FEBS Letters Année : 2002

The elastin peptides-mediated induction of pro-collagenase-1 production by human fibroblasts involves activation of MEK/ERK pathway via PKA- and PI 3 K-dependent signaling

Résumé

Elastin peptides, such as kappa-elastin (kE), bind to the elastin receptor at the cell surface of human dermal fibroblasts and stimulate collagenase-1 expression at the gene and protein levels. Using specific inhibitors and phosphospecific antibodies, we show here that the binding of elastin peptides to their receptor activates the extracellular signal-regulated kinase (ERK) pathway; this activation is essential for the induction of pro-collagenase-1 production. Moreover, protein kinase A (PKA) and phosphatidylinositol 3-kinase (PI(3)K) signaling were found to participate in ERK activation. Concomitantly, we demonstrate that stimulation by elastin peptides leads to enhanced DNA binding of activator protein-1 (AP-1). Our data indicate that the up-regulation of collagenase-1 following treatment of fibroblasts with elastin peptides results from a cross-talk between PKA, PI(3)K and the ERK signaling pathways and that this regulation is accompanied by activation of AP-1 transcription factors.

Dates et versions

hal-02340208 , version 1 (30-10-2019)

Identifiants

Citer

Laurent Duca, Laurent Debelle, Romain Debret, Frank Antonicelli, William Hornebeck, et al.. The elastin peptides-mediated induction of pro-collagenase-1 production by human fibroblasts involves activation of MEK/ERK pathway via PKA- and PI 3 K-dependent signaling. FEBS Letters, 2002, 524 (1-3), pp.193-198. ⟨10.1016/s0014-5793(02)03057-0⟩. ⟨hal-02340208⟩
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