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Article Dans Une Revue Viruses Année : 2019

Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus

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To test the importance of the host genotype in maintaining virus genetic diversity, five experimental populations were constructed by mixing two Cydia pomonella granulovirus isolates, the Mexican isolate CpGV-M and the CpGV-R5, in ratios of 99% M + 1% R, 95% M + 5% R, 90% M + 10% R, 50% M + 50% R, and 10% M + 90% R. CpGV-M and CpGV-R5 differ in their ability to replicate in codling moth larvae carrying the type I resistance. This ability is associated with a genetic marker located in the virus pe38 gene. Six successive cycles of replication were carried out with each virus population on a fully-permissive codling moth colony (CpNPP), as well as on a host colony (R GV) that carries the type I resistance, and thus blocks CpGV-M replication. The infectivity of offspring viruses was tested on both hosts. Replication on the CpNPP leads to virus lineages preserving the pe38 markers characteristic of both isolates, while replication on the R GV colony drastically reduces the frequency of the CpGV-M pe38 marker. Virus progeny obtained after replication on CpNPP show consistently higher pathogenicity than that of progeny viruses obtained by replication on R GV , independently of the host used for testing.
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hal-02338847 , version 1 (30-10-2019)

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Benoit Graillot, Christine Blachère-Lopez, Samantha Besse, Myriam Siegwart, Miguel Lopez-Ferber. Importance of the Host Phenotype on the Preservation of the Genetic Diversity in Codling Moth Granulovirus. Viruses, 2019, Special Issue : Insect Viruses and Pest Management, 11 (7), pp.art. 621. ⟨10.3390/v11070621⟩. ⟨hal-02338847⟩
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