Mannose-coated Fluorescent Lipid Microparticles for Specific Cellular Targeting and Internalization via Glycoreceptor-Induced Phagocytosis - Archive ouverte HAL Access content directly
Journal Articles ACS Applied Bio Materials Year : 2019

Mannose-coated Fluorescent Lipid Microparticles for Specific Cellular Targeting and Internalization via Glycoreceptor-Induced Phagocytosis

Abstract

In this work we report on the development of mannose-coated fluorescent lipid microparticles to study the role of C-type lectin membrane receptor in phagocytosis. The micrometric droplets of soybean oil in water emulsion were functionalized with a tailor-made fluorescent mannolipid. The amphiphilic ligand was built from a mannose unit, a lipid C11 spacer and a naphthalimide fluorophore. The droplets functionalization was monitored by fluorescence microscopy as well as the interaction with concanavalin A which was used as a model lectin in vitro. The use of a monovalent ligand on the surface of emulsion droplets yielded particles with an affinity approximately 40 times higher than that of free mannose. In cellulo, the coated droplets were shown to be specifically internalized by macrophages in a receptor-dependent phagocytic pathway. The naked droplets on the other hand display very little internalization due to their low immunogenicity. This work thus brings evidence that C-type lectin membrane receptors may act as phagocytic receptors. The droplets functionalization with the tailored amphiphilic fluorescent ligand and the droplets functionalization also provides insights into the development of organic fluorescent particles that may prove useful for developing targeted imaging and delivery tools.
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Dates and versions

hal-02338500 , version 1 (30-11-2020)

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Blaise Dumat, Lorraine Montel, Léa Pinon, Pascal Matton, Laurent Cattiaux, et al.. Mannose-coated Fluorescent Lipid Microparticles for Specific Cellular Targeting and Internalization via Glycoreceptor-Induced Phagocytosis. ACS Applied Bio Materials, 2019, ⟨10.1021/acsabm.9b00793⟩. ⟨hal-02338500⟩
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