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Article Dans Une Revue Chemistry - A European Journal Année : 2019

Structure Elucidation of Helical Aromatic Foldamer–Protein Complexes with Large Contact Surface Areas

Résumé

The development of large synthetic ligands could be useful to target the sizeable surface areas involved in protein–protein interactions. Herein, we present long helical aromatic oligoamide foldamers bearing proteinogenic side chains that cover up to 450 Å2 of the human carbonic anhydrase II (HCA) surface. The foldamers are composed of aminoquinolinecarboxylic acids bearing proteinogenic side chains and of more flexible aminomethyl‐pyridinecarboxylic acids that enhance helix handedness dynamics. Crystal structures of HCA‐foldamer complexes were obtained with a 9‐ and a 14‐mer both showing extensive protein–foldamer hydrophobic contacts. In addition, foldamer–foldamer interactions seem to be prevalent in the crystal packing, leading to the peculiar formation of an HCA superhelix wound around a rod of stacked foldamers. Solution studies confirm the positioning of the foldamer at the protein surface as well as a dimerization of the complexes.
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hal-02333661 , version 1 (24-11-2020)

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Post Sai Reddy, Béatrice Langlois d'Estaintot, Thierry Granier, Cameron Mackereth, Lucile Fischer, et al.. Structure Elucidation of Helical Aromatic Foldamer–Protein Complexes with Large Contact Surface Areas. Chemistry - A European Journal, 2019, 25 (47), pp.11042-11047. ⟨10.1002/chem.201902942⟩. ⟨hal-02333661⟩
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