Dendrimer mediated targeting of siRNA against polo‐like kinase for the treatment of triple negative breast cancer - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biomedical Materials Research Part A Année : 2019

Dendrimer mediated targeting of siRNA against polo‐like kinase for the treatment of triple negative breast cancer

Résumé

Irresponsiveness of triple negative breast cancer (TNBC) toward conventional therapies has drawn attention toward siRNA therapeutics. In gene delivery, dendrimers are gaining significant attention due to their characteristic features and polo-like kinase (PLK1) is reported as a potential target for TNBC. In this work, phosphorus and polyamidoamine dendrimer (generation 3 and 4 of each type) are explored to address delivery challenges of PLK1 siRNA (siPLK1). Dendriplexes were formed and complexation was found at 3:1 N/P ratio for all dendrimers by gel electrophoresis. Complexation was also supported by zeta potential, circular dichroism and intercalation assay. Dendriplexes were found to be stable in presence of ribonuclease and serum. Dendriplexes resulted in enhanced cell uptake of siPLK1 compared to siPLK1 solution in MDA-MB-231 and MCF-7 cells. Dendriplexes caused increased cell arrest in sub-G1 phase compared to solution. These observations suggested phosphorus and polyamidoamine dendrimers as potential carriers for siPLK1 delivery to treat TNBC.

Dates et versions

hal-02333160 , version 1 (25-10-2019)

Identifiants

Citer

Anjali Jain, Shaheen Mahira, Jean Pierre Majoral, Maria Bryszewska, Wahid Khan, et al.. Dendrimer mediated targeting of siRNA against polo‐like kinase for the treatment of triple negative breast cancer. Journal of Biomedical Materials Research Part A, 2019, 107 (9), pp.1933-1944. ⟨10.1002/jbm.a.36701⟩. ⟨hal-02333160⟩
18 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More