Susceptibility of Agar-Entrapped Escherichia coli Cultures to Cefotaxime as Assessed by the Potentiometric Measurement of Lipoic Acid Reduction - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Chemotherapy Année : 2016

Susceptibility of Agar-Entrapped Escherichia coli Cultures to Cefotaxime as Assessed by the Potentiometric Measurement of Lipoic Acid Reduction

Odile Tresse
T. Jouenne
G.A. Junter
  • Fonction : Auteur

Résumé

We used an original potentiometric procedure to monitor the metabolic activity of biofilm-like structures and to assess their susceptibility to betalactam antibiotics. The reduction of exogeneous alpha-lipoic acid to dihydrolipoic acid by planktonic and agar-entrapped bacteria, metabolizing glucose, was followed by potential-time measurements using gold versus reference electrodes. Immobilized cultures displayed a lower susceptibility to cefotaxime than their free counterparts. The redox potential-time courses showed that the gel matrix was more rapidly oxygen depleted than the free-cell culture medium. The depletion of both glucose and dissolved O2 inside the gel induced a breakdown in the reducing activity of agar-entrapped organisms.
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Dates et versions

hal-02330896 , version 1 (24-10-2019)

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Citer

Odile Tresse, T. Jouenne, G.A. Junter. Susceptibility of Agar-Entrapped Escherichia coli Cultures to Cefotaxime as Assessed by the Potentiometric Measurement of Lipoic Acid Reduction. Journal of Chemotherapy, 2016, 6 (6), pp.388-391. ⟨10.1080/1120009X.1994.11736494⟩. ⟨hal-02330896⟩
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