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Article Dans Une Revue Enzyme and Microbial Technology Année : 1994

Exopolysaccharide production by free and immobilized microbial cultures

Résumé

The batch production of different exopolysaccharides (alginate, xanthan, pullulan, dextran) by free and immobilized microbial cultures was investigated. First, conventional free-cell cultures were performed to obtain control fermentation parameters and macromolecular characteristics of exopolysaccharides. Then microbial cultures were immobilized in composite agar layer/microporous membrane structures and tested for polysaccharide production. The immobilized-cell system proved unsuitable for xanthan and pullulan production. Owing to the fouling of the microporous membrane by the polysaccharide, dextran production by immobilized Leuconostoc mesenteroides also was inefficient. More promising results have been obtained with immobilized Azotobacter vinelandii cultures. The amount of alginate produced by immobilized A. vinelandii represented about 60% of that recovered from a free-cell culture, whereas the polysaccharide yield reached 35% instead of 9% for the free counterpart. These results are compared to the macromolecular characteristics of exopolysaccharides.

Dates et versions

hal-02330808 , version 1 (24-10-2019)

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Claire Hinfray, Laurent Lebrun, Guy-Alain Junter, Thierry Jouenne, L. Mignot. Exopolysaccharide production by free and immobilized microbial cultures. Enzyme and Microbial Technology, 1994, 16 (12), pp.1048-1054. ⟨10.1016/0141-0229(94)90141-4⟩. ⟨hal-02330808⟩
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