Vaccines inducing immunity to Lassa virus glycoprotein and nucleoprotein protect macaques after a single shot
Mathieu Mateo
(1)
,
Stéphanie Reynard
(1)
,
Xavier Carnec
(1)
,
Alexandra Journeaux
(1)
,
Nicolas Baillet
(1)
,
Justine Schaeffer
(1)
,
Caroline Picard
(1)
,
Catherine Legras-Lachuer
(2)
,
Richard Allan
(2)
,
Emeline Perthame
(3)
,
Kenzo-Hugo Hillion
(3)
,
Natalia Pietrosemoli
(3)
,
Marie-Agnès Dillies
(3)
,
Laura Barrot
(4)
,
Audrey Vallve
(4)
,
Stéphane Barron
(4)
,
Lyne Fellmann
(5)
,
Jean-Charles Gaillard
(6)
,
J. Armengaud
(6)
,
Caroline Carbonnelle
(4)
,
Hervé Raoul
(4)
,
Frédéric Tangy
(7)
,
Sylvain Baize
(1)
1
UBIVE -
Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections [CIRI]
2 Viroscan3D SAS [Lyon, France]
3 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
4 Unité de service 3 - Laboratoire P4 Jean Mérieux-Inserm [Lyon]
5 UNISTRA - Université de Strasbourg
6 LI2D - Laboratoire Innovations technologiques pour la Détection et le Diagnostic
7 Génomique virale et vaccination
2 Viroscan3D SAS [Lyon, France]
3 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
4 Unité de service 3 - Laboratoire P4 Jean Mérieux-Inserm [Lyon]
5 UNISTRA - Université de Strasbourg
6 LI2D - Laboratoire Innovations technologiques pour la Détection et le Diagnostic
7 Génomique virale et vaccination
Mathieu Mateo
- Fonction : Auteur
- PersonId : 177265
- IdHAL : mathieu-mateo
- ORCID : 0000-0001-7941-0245
- IdRef : 149859708
Stéphanie Reynard
- Fonction : Auteur
- PersonId : 184881
- IdHAL : stephanie-reynard
- ORCID : 0000-0002-8508-5167
Xavier Carnec
- Fonction : Auteur
- PersonId : 1213299
- ORCID : 0000-0001-7858-1675
Emeline Perthame
- Fonction : Auteur
- PersonId : 185133
- IdHAL : emeline-perthame
- ORCID : 0000-0002-8266-5908
- IdRef : 193507846
Natalia Pietrosemoli
- Fonction : Auteur
- PersonId : 734724
- IdHAL : natalia-pietrosemoli
Marie-Agnès Dillies
- Fonction : Auteur
- PersonId : 746907
- IdHAL : marie-agnes-dillies
- ORCID : 0000-0001-9487-2624
J. Armengaud
- Fonction : Auteur
- PersonId : 740098
- IdHAL : jean-armengaud
- ORCID : 0000-0003-1589-445X
- IdRef : 133877280
Frédéric Tangy
- Fonction : Auteur
- PersonId : 755793
- ORCID : 0000-0002-5937-5654
- IdRef : 087529696
Sylvain Baize
Connectez-vous pour contacter l'auteur
- Fonction : Auteur correspondant
- PersonId : 746348
- IdHAL : sylvain-baize
- ORCID : 0000-0002-3234-7477
- IdRef : 129432164
Connectez-vous pour contacter l'auteur
Résumé
Lassa fever is a major threat in Western Africa. The large number of people living at risk for this disease calls for the development of a vaccine against Lassa virus (LASV). We generated live-attenuated LASV vaccines based on measles virus and Mopeia virus platforms and expressing different LASV antigens, with the aim to develop a vaccine able to protect after a single shot. We compared the efficacy of these vaccines against LASV in cynomolgus monkeys. The vaccines were well tolerated and protected the animals from LASV infection and disease after a single immunization but with varying efficacy. Analysis of the immune responses showed that complete protection was associated with robust secondary T cell and antibody responses against LASV. Transcriptomic and proteomic analyses showed an early activation of innate immunity and T cell priming after immunization with the most effective vaccines, with changes detectable as early as 2 days after immunization. The most efficacious vaccine candidate, a measles vector simultaneously expressing LASV glycoprotein and nucleoprotein, has been selected for further clinical evaluation.