Vaccines inducing immunity to Lassa virus glycoprotein and nucleoprotein protect macaques after a single shot - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Science Translational Medicine Année : 2019

Vaccines inducing immunity to Lassa virus glycoprotein and nucleoprotein protect macaques after a single shot

Frédéric Tangy

Résumé

Lassa fever is a major threat in Western Africa. The large number of people living at risk for this disease calls for the development of a vaccine against Lassa virus (LASV). We generated live-attenuated LASV vaccines based on measles virus and Mopeia virus platforms and expressing different LASV antigens, with the aim to develop a vaccine able to protect after a single shot. We compared the efficacy of these vaccines against LASV in cynomolgus monkeys. The vaccines were well tolerated and protected the animals from LASV infection and disease after a single immunization but with varying efficacy. Analysis of the immune responses showed that complete protection was associated with robust secondary T cell and antibody responses against LASV. Transcriptomic and proteomic analyses showed an early activation of innate immunity and T cell priming after immunization with the most effective vaccines, with changes detectable as early as 2 days after immunization. The most efficacious vaccine candidate, a measles vector simultaneously expressing LASV glycoprotein and nucleoprotein, has been selected for further clinical evaluation.

Dates et versions

hal-02315865 , version 1 (14-10-2019)

Identifiants

Citer

Mathieu Mateo, Stéphanie Reynard, Xavier Carnec, Alexandra Journeaux, Nicolas Baillet, et al.. Vaccines inducing immunity to Lassa virus glycoprotein and nucleoprotein protect macaques after a single shot. Science Translational Medicine, 2019, 11 (512), pp.eaaw3163. ⟨10.1126/scitranslmed.aaw3163⟩. ⟨hal-02315865⟩
197 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More