<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-02309906</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-12T04:43:06+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">A Multiparametric and High-Throughput Assay to Quantify the Influence of Target Size on Phagocytosis</title>
            <author role="aut">
              <persName>
                <forename type="first">Lorraine</forename>
                <surname>Montel</surname>
              </persName>
              <email type="md5">655343ae1db4df95c3a42cca5ebfab1c</email>
              <email type="domain">univ-lyon1.fr</email>
              <idno type="idhal" notation="string">lorraine-montel</idno>
              <idno type="idhal" notation="numeric">1175820</idno>
              <idno type="halauthorid" notation="string">781690-1175820</idno>
              <affiliation ref="#struct-541777"/>
              <affiliation ref="#struct-460255"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Léa</forename>
                <surname>Pinon</surname>
              </persName>
              <idno type="halauthorid">1667672-0</idno>
              <affiliation ref="#struct-541777"/>
              <affiliation ref="#struct-460255"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Jacques</forename>
                <surname>Fattaccioli</surname>
              </persName>
              <email type="md5">faca9ff8163c5f5cde8e4c3da927c377</email>
              <email type="domain">ens.fr</email>
              <idno type="idhal" notation="string">jacques-fattaccioli</idno>
              <idno type="idhal" notation="numeric">177876</idno>
              <idno type="halauthorid" notation="string">7859-177876</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-0095-2576</idno>
              <idno type="IDREF">https://www.idref.fr/116337222</idno>
              <affiliation ref="#struct-541777"/>
              <affiliation ref="#struct-460255"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Accord Elsevier</forename>
                <surname>CCSD</surname>
              </persName>
              <email type="md5">3cc0cc7b8e3329bfb7423fa2578d788e</email>
              <email type="domain">ccsd.cnrs.fr</email>
            </editor>
            <funder ref="#projanr-37785"/>
            <funder ref="#projanr-45472"/>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2021-12-20 16:40:37</date>
              <date type="whenModified">2025-08-12 18:12:52</date>
              <date type="whenReleased">2021-12-20 16:40:39</date>
              <date type="whenProduced">2019-08</date>
              <date type="whenEndEmbargoed">2020-08-06</date>
              <ref type="file" target="https://hal.science/hal-02309906v1/document">
                <date notBefore="2020-08-06"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.science/hal-02309906v1/file/S0006349519305107.pdf" id="file-3497663-3061294">
                <date notBefore="2020-08-06"/>
              </ref>
              <ref type="externalLink" target="http://www.cell.com/article/S0006349519305107/pdf"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="1081876">
                <persName>
                  <forename>Accord Elsevier</forename>
                  <surname>CCSD</surname>
                </persName>
                <email type="md5">3cc0cc7b8e3329bfb7423fa2578d788e</email>
                <email type="domain">ccsd.cnrs.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-02309906</idno>
            <idno type="halUri">https://hal.science/hal-02309906</idno>
            <idno type="halBibtex">montel:hal-02309906</idno>
            <idno type="halRefHtml">&lt;i&gt;Biophysical Journal&lt;/i&gt;, 2019, 117 (3), pp.408-419. &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.bpj.2019.06.021"&gt;&amp;#x27E8;10.1016/j.bpj.2019.06.021&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Biophysical Journal, 2019, 117 (3), pp.408-419. &amp;#x27E8;10.1016/j.bpj.2019.06.021&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://creativecommons.org/licenses/by-nc/4.0/">CC BY-NC 4.0 - Attribution - Non-commercial use<ref corresp="#file-3497663-3061294"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="ENS-PARIS">Ecole Normale Supérieure de Paris</idno>
            <idno type="stamp" n="ESPCI">ESPCI Paris-PSL</idno>
            <idno type="stamp" n="ENSCP" corresp="PARISTECH">Chimie ParisTech</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="ENSC-PARIS">Ecole Nationale Supérieure de Chimie de Paris</idno>
            <idno type="stamp" n="FNCLCC" corresp="INSERM">UNICANCER</idno>
            <idno type="stamp" n="CURIE" corresp="INSERM">Institut Curie</idno>
            <idno type="stamp" n="PASTEUR_UMR8640" corresp="SORBONNE-UNIVERSITE">Processus d'activation sélective par transfert d'énergie uni-électronique ou radiatif</idno>
            <idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno>
            <idno type="stamp" n="PSL">Université Paris sciences et lettres</idno>
            <idno type="stamp" n="SORBONNE-UNIVERSITE">Sorbonne Université</idno>
            <idno type="stamp" n="SORBONNE-UNIV" corresp="SORBONNE-UNIVERSITE">Sorbonne Université 01/01/2018</idno>
            <idno type="stamp" n="SU-SCIENCES" corresp="SORBONNE-UNIVERSITE">Faculté des Sciences de Sorbonne Université</idno>
            <idno type="stamp" n="TEST-HALCNRS">Collection test HAL CNRS</idno>
            <idno type="stamp" n="ENSCP-PSL" corresp="PSL">Chimie ParisTech - PSL</idno>
            <idno type="stamp" n="ENS-PSL" corresp="PSL">École normale supérieure - PSL</idno>
            <idno type="stamp" n="ESPCI-PSL" corresp="PSL">Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris - PSL</idno>
            <idno type="stamp" n="INSTITUT-CURIE-PSL" corresp="PSL">Institut Curie - PSL</idno>
            <idno type="stamp" n="SU-TI">Sorbonne Université - Texte Intégral</idno>
            <idno type="stamp" n="ANR">ANR</idno>
            <idno type="stamp" n="MSC-LAB">Laboratoire Matière et Systèmes Complexes</idno>
            <idno type="stamp" n="ELSEVIER">Import Elsevier</idno>
            <idno type="stamp" n="ALLIANCE-SU"> Alliance Sorbonne Université</idno>
            <idno type="stamp" n="TEST2-HALCNRS">TEST2-HALCNRS</idno>
            <idno type="stamp" n="CHIMIE-SU" corresp="SORBONNE-UNIVERSITE">UFR de Chimie de Sorbonne-Université</idno>
            <idno type="stamp" n="SUPRA_PHYS_CHIMIE">Physico-Chimie</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">A Multiparametric and High-Throughput Assay to Quantify the Influence of Target Size on Phagocytosis</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Lorraine</forename>
                    <surname>Montel</surname>
                  </persName>
                  <email type="md5">655343ae1db4df95c3a42cca5ebfab1c</email>
                  <email type="domain">univ-lyon1.fr</email>
                  <idno type="idhal" notation="string">lorraine-montel</idno>
                  <idno type="idhal" notation="numeric">1175820</idno>
                  <idno type="halauthorid" notation="string">781690-1175820</idno>
                  <affiliation ref="#struct-541777"/>
                  <affiliation ref="#struct-460255"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Léa</forename>
                    <surname>Pinon</surname>
                  </persName>
                  <idno type="halauthorid">1667672-0</idno>
                  <affiliation ref="#struct-541777"/>
                  <affiliation ref="#struct-460255"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Jacques</forename>
                    <surname>Fattaccioli</surname>
                  </persName>
                  <email type="md5">faca9ff8163c5f5cde8e4c3da927c377</email>
                  <email type="domain">ens.fr</email>
                  <idno type="idhal" notation="string">jacques-fattaccioli</idno>
                  <idno type="idhal" notation="numeric">177876</idno>
                  <idno type="halauthorid" notation="string">7859-177876</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-0095-2576</idno>
                  <idno type="IDREF">https://www.idref.fr/116337222</idno>
                  <affiliation ref="#struct-541777"/>
                  <affiliation ref="#struct-460255"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">11206</idno>
                <idno type="issn">0006-3495</idno>
                <idno type="eissn">1542-0086</idno>
                <title level="j">Biophysical Journal</title>
                <imprint>
                  <publisher>Biophysical Society</publisher>
                  <biblScope unit="volume">117</biblScope>
                  <biblScope unit="issue">3</biblScope>
                  <biblScope unit="pp">408-419</biblScope>
                  <date type="datePub">2019-08</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1016/j.bpj.2019.06.021</idno>
              <idno type="pii">S0006-3495(19)30510-7</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="phys.phys.phys-bio-ph">Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Phagocytosis by macrophages represents a fundamental process essential for both immunity and tissue homeostasis. It consists in the uptake of pathogenic or cellular targets larger than 0.5 μm. For the biggest particles, the phagocytic process involves a massive reorganization of membrane and actin cytoskeleton as well as an important intracellular deformation all in a matter of minutes. The study of the role of the size of objects in their phagocytosis has led to contradictory results in the last decades. We designed a method using confocal microscopy, automated image analysis, and databases for fast quantitative analysis of phagocytosis assays. It yields comprehensive data on the cells and targets geometric and fluorescence intensity parameters, automatically discriminates internalized from external targets, and stores the relationship between a cell and the targets it has engulfed. We used two types of targets (solid polystyrene beads and liquid lipid droplets) to investigate the influence of size on the phagocytic uptake of macrophages. The method made it possible not only to perform phagocytic assays with functionalized droplets and beads of different sizes but to use polydisperse particles to further our understanding of the role of size in phagocytosis. The use of monodisperse and polydisperse objects shows that whereas smaller monodisperse objects are internalized in greater numbers, objects of different sizes presented simultaneously are internalized without preferred size. The total surface engulfed by the cell is thus the main factor limiting the uptake of particles, regardless of their nature or size. A meta-analysis of the literature reveals that this dependence in surface is consistently conserved throughout cell types, targets’ nature, or activated receptors.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-541777" status="OLD">
          <idno type="IdRef">225309831</idno>
          <idno type="ISNI">0000000403689974</idno>
          <idno type="RNSR">199812965F</idno>
          <idno type="ROR">https://ror.org/033t7vz72</idno>
          <orgName>Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640)</orgName>
          <orgName type="acronym">PASTEUR</orgName>
          <date type="start">2000-01-01</date>
          <date type="end">2024-12-31</date>
          <desc>
            <address>
              <addrLine>24 Rue Lhomond 75231 PARIS CEDEX 05</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.chimie.ens.fr/?q=pasteur</ref>
          </desc>
          <listRelation>
            <relation active="#struct-240928" type="direct"/>
            <relation active="#struct-59704" type="indirect"/>
            <relation active="#struct-564132" type="indirect"/>
            <relation active="#struct-341038" type="direct"/>
            <relation active="#struct-413221" type="direct"/>
            <relation name="UMR8640" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="regrouplaboratory" xml:id="struct-460255" status="VALID">
          <idno type="ROR">https://ror.org/00q8h6763</idno>
          <orgName>Institut Pierre-Gilles de Gennes pour la Microfluidique</orgName>
          <orgName type="acronym">IPGG</orgName>
          <date type="start">2006-01-01</date>
          <desc>
            <address>
              <addrLine>ESPCI, ENS, Institut Curie, Chimie Paris, PSL 6 Rue Jean Calvin, 75005 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.institut-pgg.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-59704" type="direct"/>
            <relation active="#struct-564132" type="indirect"/>
            <relation active="#struct-300372" type="direct"/>
            <relation active="#struct-301585" type="direct"/>
            <relation active="#struct-301888" type="direct"/>
            <relation active="#struct-303623" type="direct"/>
            <relation active="#struct-413221" type="direct"/>
            <relation active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="regrouplaboratory" xml:id="struct-240928" status="VALID">
          <idno type="IdRef">198500114</idno>
          <orgName>Département de Chimie - ENS-PSL</orgName>
          <date type="start">1985-01-01</date>
          <desc>
            <address>
              <addrLine>24 rue Lhomond75132 Paris Cedex 05</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.chimie.ens.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-59704" type="direct"/>
            <relation active="#struct-564132" type="indirect"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-59704" status="VALID">
          <idno type="IdRef">031738419</idno>
          <idno type="ISNI">0000000123532622</idno>
          <idno type="ROR">https://ror.org/05a0dhs15</idno>
          <orgName>École normale supérieure - Paris</orgName>
          <orgName type="acronym">ENS-PSL</orgName>
          <date type="start">1985-07-24</date>
          <desc>
            <address>
              <addrLine>45, Rue d'Ulm - 75230 Paris cedex 05</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.ens.psl.eu/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-564132" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-564132" status="VALID">
          <idno type="IdRef">241597595</idno>
          <idno type="ISNI">0000 0004 1784 3645</idno>
          <idno type="ROR">https://ror.org/013cjyk83</idno>
          <orgName>Université Paris Sciences et Lettres</orgName>
          <orgName type="acronym">PSL</orgName>
          <desc>
            <address>
              <addrLine>60 rue Mazarine 75006 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.psl.eu/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-341038" status="VALID">
          <idno type="IdRef">232435103</idno>
          <idno type="ISNI">000000010626358X</idno>
          <idno type="ROR">https://ror.org/02cte4b68</idno>
          <orgName>Institut de Chimie - CNRS Chimie</orgName>
          <orgName type="acronym">INC-CNRS</orgName>
          <date type="start">2009-10-01</date>
          <desc>
            <address>
              <addrLine>CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE3 rue Michel-Ange75794 PARIS CEDEX 16 - France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cnrs.fr/inc</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-413221" status="VALID">
          <idno type="IdRef">221333754</idno>
          <idno type="ROR">https://ror.org/02en5vm52</idno>
          <orgName>Sorbonne Université</orgName>
          <orgName type="acronym">SU</orgName>
          <date type="start">2018-01-01</date>
          <desc>
            <address>
              <addrLine>21 rue de l’École de médecine - 75006 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.sorbonne-universite.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-300372" status="VALID">
          <idno type="ROR">https://ror.org/05q65zh81</idno>
          <orgName>Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL</orgName>
          <orgName type="acronym">ENSCP</orgName>
          <date type="start">2016-09-15</date>
          <desc>
            <address>
              <addrLine>11, rue Pierre et Marie Curie75231 Paris Cedex 05</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.chimie-paristech.fr/fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-564132" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-301585" status="VALID">
          <idno type="IdRef">028114779</idno>
          <idno type="ISNI">000000009591493X</idno>
          <idno type="ROR">https://ror.org/03zx86w41</idno>
          <orgName>Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris</orgName>
          <orgName type="acronym">ESPCI Paris</orgName>
          <date type="start">1882-10-01</date>
          <desc>
            <address>
              <addrLine>10 rue Vauquelin, 75231 Paris cedex 05</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.espci.psl.eu/fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-564132" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-301888" status="VALID">
          <idno type="ISNI">0000 0004 0639 6384</idno>
          <idno type="ROR">https://ror.org/04t0gwh46</idno>
          <orgName>Institut Curie [Paris]</orgName>
          <desc>
            <address>
              <addrLine>26 rue d'Ulm - 75248 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://science.curie.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="anrProject" xml:id="projanr-37785" status="VALID">
          <idno type="anr">ANR-10-IDEX-0001</idno>
          <idno type="program">Initiative d'excellence</idno>
          <orgName>PSL</orgName>
          <desc>Paris Sciences et Lettres</desc>
          <date type="start">2010</date>
        </org>
        <org type="anrProject" xml:id="projanr-45472" status="VALID">
          <idno type="anr">ANR-15-CE18-0014</idno>
          <orgName>PhagoDrop</orgName>
          <desc>Phagocytose de gouttes d'émulsions fonctionnalisées par des IgG</desc>
          <date type="start">2015</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>